Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction

Ryouji Karinaga, Kazuya Koumoto, Jusaku Minari, Masami Mizu, Takahisa Anada, Seiji Shinkai, Kazuo Sakurai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A single chain of schizophyllan (β-(1→3)-D-glucan) formed a complex with a homo-polynucleotide. In this paper, using adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations.

Original languageEnglish
Title of host publication54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan
Pages2309
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
Country/TerritoryJapan
CityYokohama
Period5/25/055/27/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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