Competitive ELISA-like label-free detection of lysozyme by using a fluorescent monomer-doped molecularly imprinted hydrogel

Takuya Kubo, Shunsuke Arimura, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We report on a molecularly imprinted hydrogel for the selective fluorescent detection of a targeting protein. To achieve the fluorescent detection of proteins, a fluorescent functional monomer was synthesized with fluorescein isothiocyanate and allylamine. The monomer (FITC-AA) provided a gradable alteration of the absorption spectrum, and a decrease of the fluorescent intensity due to an interaction with the model protein, lysozyme. Also, the imprinted hydrogel (PI-gel) was simply prepared with FITC-AA, the anionic functional monomers, poly(ethylene glycol) dimethacrylate, and lysozyme by radical photo polymerization. The PI-gel showed selective adsorption for lysozyme and the imprinting factor reached to 2.7. Additionally, the PI-gel indicated a gradual decrease of the fluorescent intensity corresponding to the lysozyme concertation similar to a competitive enzyme-linked immunosorbent assay detection.

Original languageEnglish
Pages (from-to)1311-1315
Number of pages5
Journalanalytical sciences
Volume33
Issue number11
DOIs
Publication statusPublished - 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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