TY - JOUR
T1 - Complement activation by polymers carrying hydroxyl groups
AU - Arima, Yusuke
AU - Kawagoe, Masako
AU - Toda, Mitsuaki
AU - Iwata, Hiroo
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2009/10/28
Y1 - 2009/10/28
N2 - Hydrogels of polymers carrying surface hydroxyl groups strongly activate the complement system through the alternative pathway, although it has also been reported that solutions of polymers do not. To address these curious, inconsistent results, we examined the effect of polymer states, either immobilized on a surface or soluble in serum, on the complement activation using a surface plasmon resonance apparatus and enzyme-linked immunosorbent assay. We clearly showed that dextran- and poly(vinyl alcohol)-immobilized surfaces strongly activated the complement system but that soluble polymers could not, even when the amounts of the soluble polymers added to serum were 4-2000 times higher than those on the polymer-immobilized surfaces.
AB - Hydrogels of polymers carrying surface hydroxyl groups strongly activate the complement system through the alternative pathway, although it has also been reported that solutions of polymers do not. To address these curious, inconsistent results, we examined the effect of polymer states, either immobilized on a surface or soluble in serum, on the complement activation using a surface plasmon resonance apparatus and enzyme-linked immunosorbent assay. We clearly showed that dextran- and poly(vinyl alcohol)-immobilized surfaces strongly activated the complement system but that soluble polymers could not, even when the amounts of the soluble polymers added to serum were 4-2000 times higher than those on the polymer-immobilized surfaces.
UR - http://www.scopus.com/inward/record.url?scp=77954384182&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954384182&partnerID=8YFLogxK
U2 - 10.1021/am9005463
DO - 10.1021/am9005463
M3 - Article
C2 - 20355878
AN - SCOPUS:77954384182
VL - 1
SP - 2400
EP - 2407
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 10
ER -