Complement activation on surfaces modified with ethylene glycol units

Yusuke Arima, Mitsuaki Toda, Hiroo Iwata

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Poly(ethylene glycol) (PEG) has been widely used for the preparation of biomedical devices and drug carriers to reduce the interaction of proteins with artificial materials. However, unanticipated body reactions such as hypersensitivity reactions caused by PEG-modified surfaces have been reported. Body reactions to PEG-modified materials still remain unclear. In this study, complement activation on surfaces modified with ethylene glycol units was examined. Two surfaces modified with tri(ethylene glycol)-terminated alkanethiol (HS-TEGOH) and methoxy-terminated PEG-thiol (HS-mPEG) were employed as model surfaces. Complement activation was assessed by the binding of an antibody against complement C3b after exposure to diluted human serum using a surface plasmon resonance (SPR) apparatus. Strong complement activation was observed on HS-TEGOH but not on HS-mPEG surfaces. The terminal hydroxyl group of HS-TEGOH is involved in complement activation. Although the HS-mPEG surface just after preparation did not induce complement activation, it became a stronger activator with storage period under room light at room temperature. Complement activation on the HS-mPEG surface was further accelerated by irradiation with UV light. These results suggest that functional groups which can activate the complement system are introduced onto the HS-mPEG surface by oxidation during long-term storage and UV irradiation.

Original languageEnglish
Pages (from-to)551-560
Number of pages10
JournalBiomaterials
Volume29
Issue number5
DOIs
Publication statusPublished - Feb 1 2008
Externally publishedYes

Fingerprint

Ethylene Glycol
Complement Activation
Ethylene glycol
Polyethylene glycols
Sulfhydryl Compounds
Chemical activation
Complement C3b
Drug Carriers
Surface Plasmon Resonance
Ultraviolet Rays
Irradiation
Hydroxyl Radical
Hypersensitivity
Surface plasmon resonance
Light
Antibodies
Ultraviolet radiation
Equipment and Supplies
Functional groups
Temperature

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Complement activation on surfaces modified with ethylene glycol units. / Arima, Yusuke; Toda, Mitsuaki; Iwata, Hiroo.

In: Biomaterials, Vol. 29, No. 5, 01.02.2008, p. 551-560.

Research output: Contribution to journalArticle

Arima, Yusuke ; Toda, Mitsuaki ; Iwata, Hiroo. / Complement activation on surfaces modified with ethylene glycol units. In: Biomaterials. 2008 ; Vol. 29, No. 5. pp. 551-560.
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