Complementary DNA cloning and characterization of rat spergen-2, a spermatogenic cell-specific gene 2 encoding a 56-kilodalton nuclear protein bearing ankyrin repeat motifs

Hiroshi Iida, Aiko Urasoko, Masamichi Doiguchi, Takayuki Mori, Kiyotaka Toshimori, Yosaburo Shibata

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Differential display in combination with a cDNA cloning approach were used to isolate a novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes a protein of 500 amino acids that contains ankyrin repeat motifs and a putative nuclear localization signal. Expression of spergen-2 is developmentally upregulated in testis. In situ hybridization revealed that spergen-2 mRNA is expressed in spermatocytes and round spermatids (steps 1-6). Immunohistochemical analysis with confocal laser-scanning microscopy demonstrated that spergen-2 protein is predominantly expressed in nuclei of late spermatocytes (stages IX-XIV) and spermatids (steps 1-11), indicating the restricted expression of spergen-2 during spermatogenesis. In nucleoplasm of spermatogenic cell nuclei, spergen-2 tends to localize in the interchromosome space with relatively low DNA density. These findings indicate a potential role of spergen-2 in spermatogenesis, especially in cell differentiation from late pachytene spermatocytes to spermatids or in early spermatid differentiation.

Original languageEnglish
Pages (from-to)421-429
Number of pages9
JournalBiology of reproduction
Volume69
Issue number2
DOIs
Publication statusPublished - Aug 1 2003

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

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