Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase

C. Aoyama, K. Nakashima, M. Matsui, K. Ishidate

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

By means of peptide sequence information, several cDNA clones encoding a 42 kDa choline/ethanolamine kinase were isolated from a rat kidney cDNA library. Eight clones were sequenced with all of them resulting in identical overlapping nucleotide sequences. Four of them possessed entire open reading frame which could encode 394 amino acids with a calculated molecular size of 45,100. The predicted amino acid sequence contained all of the internal peptide fragment sequences derived from the purified 42 kDa enzyme. When the open reading frame was introduced into pGEX-2T vector and transfected into E. coli cells, a significant choline/ethanolamine kinase activity did appear in the cell lysate. A homology comparison with the previously reported choline kinase cDNAs (CKR1 and CKR2) from rat liver showed 66%-68% in entire nucleotide sequences and 57%-59% in amino acid sequences, indicating that the cloned cDNA here must be a novel CK/EK gene product.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1390
Issue number1
DOIs
Publication statusPublished - Feb 5 1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Endocrinology

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