Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer

Yuka Okada, Yuko Ueshin, Ayako Isotani, Tomoko Saito-Fujita, Hisako Nakashima, Kazushi Kimura, Akira Mizoguchi, Masatsugu Ohora, Yoshiko Mori, Masato Ogata, Robert G. Oshima, Masaru Okabe, Masahito Ikawa

    Research output: Contribution to journalArticlepeer-review

    78 Citations (Scopus)

    Abstract

    Placental dysfunction underlies many complications during pregnancy, and better understanding of gene function during placentation could have considerable clinical relevance. However, the lack of a facile method for placenta-specific gene manipulation has hampered investigation of placental organogenesis and the treatment of placental dysfunction. We showed previously that transduction of fertilized mouse eggs with lentiviral vectors leads to transgene expression in both the fetus and the placenta. Here we report placenta-specific gene incorporation by lentiviral transduction of mouse blastocysts after removal of the zona pellucida. All of the placentas analyzed, but none of the fetuses, were transgenic. Application of this method substantially rescued mice deficient in Ets2, Mapk14 (also known as p38α) and Mapk1 (also known as Erk2) from embryonic lethality caused by placental defects. Ectopic expression of Mapk11 also complemented Mapk14 deficiency during placentation.

    Original languageEnglish
    Pages (from-to)233-237
    Number of pages5
    JournalNature Biotechnology
    Volume25
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2007

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Molecular Medicine
    • Biomedical Engineering

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