Complete amino acid sequence of chitinase-a from leaves of pokeweed (phytolacca americana)

Takeshi Yamagami, Miho Tanigawa, Masatsune Ishiguro, Gunki Funatsu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The complete amino acid sequence of pokeweed leaf chitinase-A was determined. First all 11 tryptic peptides from the reduced and S-carboxymethylated form of the enzyme were sequenced. Then the same form of the enzyme was cleaved with cyanogen bromide, giving three fragments. The fragments were digested with chymotrypsin or Staphylococcus aureus V8 protease. Last, the 11 tryptic peptides were put in order. Of seven cysteine residues, six were linked by disulfide bonds (between Cys25 and Cys74, Cys89 and Cys98, and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain without a chitin-binding domain. The length of the chain was almost the same as that of the catalytic domains of class IL chitinases. These findings suggested that this enzyme is a new kind of class IIL chitinase, although its sequence resembles that of catalytic domains of class IL chitinases more than that of the class IIL chitinases reported so far. Discussion on the involvement of specific tryptophan residue in the active site of PLC-A is also given based on the sequence similarity with rye seed chitinase-c.

Original languageEnglish
Pages (from-to)825-828
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number4
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Phytolacca americana
Chitinases
Amino Acid Sequence
Amino Acids
Catalytic Domain
Enzymes
Peptides
Cyanogen Bromide
Chitin
Chymotrypsin
Programmable logic controllers
Tryptophan
Disulfides
Cysteine
Seed
Staphylococcus aureus
Seeds
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Complete amino acid sequence of chitinase-a from leaves of pokeweed (phytolacca americana). / Yamagami, Takeshi; Tanigawa, Miho; Ishiguro, Masatsune; Funatsu, Gunki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 4, 01.01.1998, p. 825-828.

Research output: Contribution to journalArticle

Yamagami, Takeshi ; Tanigawa, Miho ; Ishiguro, Masatsune ; Funatsu, Gunki. / Complete amino acid sequence of chitinase-a from leaves of pokeweed (phytolacca americana). In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 4. pp. 825-828.
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