Complete amino acid sequences of three proteinase inhibitors from white sword bean (canavalia gladiata)

Sung Soo Park, Toshihisa Sumi, Hideki Ohba, Osamu Nakamura, Makoto Kimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Three major serine proteinase inhibitors (SBI-1, -2, and -3) were purified from the seeds of white sword bean (Canavalia gladiata) by FPLC and reversed-phase HPLC. The sequences of these inhibitors were established by automatic Edman degradation and TOF-mass spectrometry. SBI-1, -2, and -3 consisted of 72, 73, and 75 amino acid residues, with molecular masses of 7806.5, 7919.8, and 8163.4, respectively. The sequences of SBI-1 and -2 coincided with those of CLT I and II [Terada et al. (1994) Biosci. Biotech. Biochem., 58, 376-379] except only N- or C-terminal amino acid residues. Analysis of the amino acid sequences showed that the active sites of the inhibitors contained a Lys21-Ser22 against trypsin and Leu48-Ser49 against chymotrypsin, respectively. Further, it became apparent that about seven disulfide bonds were present. These results suggest that sword bean inhibitors are members of the Bowman-Birk proteinase inhibitor family.

Original languageEnglish
Pages (from-to)2272-2275
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number10
DOIs
Publication statusPublished - Jan 1 2000

Fingerprint

Canavalia
Amino Acid Sequence
Peptide Hydrolases
Amino Acids
Serine Proteinase Inhibitors
Protein Sequence Analysis
Chymotrypsin
Disulfides
Trypsin
Mass Spectrometry
Catalytic Domain
Seeds
High Pressure Liquid Chromatography
Molecular mass
Corrosion inhibitors
Mass spectrometry
Seed
Degradation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Complete amino acid sequences of three proteinase inhibitors from white sword bean (canavalia gladiata). / Park, Sung Soo; Sumi, Toshihisa; Ohba, Hideki; Nakamura, Osamu; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 10, 01.01.2000, p. 2272-2275.

Research output: Contribution to journalArticle

Park, Sung Soo ; Sumi, Toshihisa ; Ohba, Hideki ; Nakamura, Osamu ; Kimura, Makoto. / Complete amino acid sequences of three proteinase inhibitors from white sword bean (canavalia gladiata). In: Bioscience, Biotechnology and Biochemistry. 2000 ; Vol. 64, No. 10. pp. 2272-2275.
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