Complete genome sequence of Enterococcus mundtii QU 25, an efficient L-(+)-lactic acid-producing bacterium

Shiwa Yuh, Yanase Hiroaki, Hirose Yuu, Satomi Shohei, Araya Kojima Tomoko, Watanabe Satoru, Zendo Takeshi, Chibazakura Taku, Shimizu Kadota Mariko, Yoshikawa Hirofumi, Sonomoto Kenji

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12 Citations (Scopus)

Abstract

Enterococcus mundtii QU 25, a non-dairy bacterial strain of ovine faecal origin, can ferment both cellobiose and xylose to produce L-lactic acid. The use of this strain is highly desirable for economical L-lactate production from renewable biomass substrates. Genome sequence determination is necessary for the genetic improvement of this strain. We report the complete genome sequence of strain QU 25, primarily determined using Pacific Biosciences sequencing technology. The E. mundtii QU 25 genome comprises a 3 022 186-bp single circular chromosome (GC content, 38.6%) and five circular plasmids: pQY182, pQY082, pQY039, pQY024, and pQY003. In all, 2900 protein-coding sequences, 63 tRNA genes, and 6 rRNA operonswere predicted in theQU25chromosome. Plasmid pQY024 harbours genes for mundticin production. We found that strain QU 25 produces a bacteriocin, suggesting that mundticin-encoded genes on plasmid pQY024 were functional. For lactic acid fermentation, two gene clusters were identified - one involved in the initial metabolism of xylose and uptake of pentose and the second containing genes for the pentose phosphate pathway and uptake of related sugars. This is the first complete genome sequence of an E. mundtii strain. The data provide insights into lactate production in this bacterium and its evolution among enterococci.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalDNA Research
Volume21
Issue number4
DOIs
Publication statusPublished - Aug 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Yuh, S., Hiroaki, Y., Yuu, H., Shohei, S., Tomoko, A. K., Satoru, W., Takeshi, Z., Taku, C., Mariko, S. K., Hirofumi, Y., & Kenji, S. (2014). Complete genome sequence of Enterococcus mundtii QU 25, an efficient L-(+)-lactic acid-producing bacterium. DNA Research, 21(4), 369-377. https://doi.org/10.1093/dnares/dsu003