Complete in vitro generation of fertile oocytes from mouse primordial germ cells

Kanako Morohaku, Ren Tanimoto, Keisuke Sasaki, Ryouka Kawahara-Miki, Tomohiro Kono, Katsuhiko Hayashi, Yuji Hirao, Yayoi Obata

Research output: Contribution to journalArticle

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Abstract

Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful in vitro reconstitution of primordial germ cells and spermatogenesis has recently had a significant effect in the field. However, recapitulation of oogenesis in vitro remains unachieved. Here we demonstrate the first reconstitution, to our knowledge, of the entire process of mammalian oogenesis in vitro from primordial germ cells, using an estrogenreceptor antagonist that promotes normal follicle formation, which in turn is crucial for supporting oocyte growth. The fundamental events in oogenesis (i.e., meiosis, oocyte growth, and genomic imprinting) were reproduced in the culture system. The most rigorous evidence of the recapitulation of oogenesis was the birth of fertile offspring, with a maximum of seven pups obtained from a cultured gonad. Moreover, cryopreserved gonads yielded functional oocytes and offspring in this culture system. Thus, our in vitro system will enable both innovative approaches for a deeper understanding of oogenesis and a new avenue to create and preserve female germ cells.

Original languageEnglish
Pages (from-to)9021-9026
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number32
DOIs
Publication statusPublished - Aug 9 2016

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Oogenesis
Germ Cells
Oocytes
Gonads
Gametogenesis
Genomic Imprinting
Reproductive Medicine
Regenerative Medicine
Meiosis
Spermatogenesis
Growth
In Vitro Techniques
Parturition

All Science Journal Classification (ASJC) codes

  • General

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Complete in vitro generation of fertile oocytes from mouse primordial germ cells. / Morohaku, Kanako; Tanimoto, Ren; Sasaki, Keisuke; Kawahara-Miki, Ryouka; Kono, Tomohiro; Hayashi, Katsuhiko; Hirao, Yuji; Obata, Yayoi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 32, 09.08.2016, p. 9021-9026.

Research output: Contribution to journalArticle

Morohaku, Kanako ; Tanimoto, Ren ; Sasaki, Keisuke ; Kawahara-Miki, Ryouka ; Kono, Tomohiro ; Hayashi, Katsuhiko ; Hirao, Yuji ; Obata, Yayoi. / Complete in vitro generation of fertile oocytes from mouse primordial germ cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 32. pp. 9021-9026.
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