Complete nucleotide sequence of plasmid Rts1: Implications for evolution of large plasmid genomes

Takahiro Murata, Makoto Ohnishi, Takeshi Ara, Jun Kaneko, Chang Gyun Han, Yong Fang Li, Kayoko Takashima, Hideaki Nojima, Keisuke Nakayama, Akira Kaji, Yoshiyuki Kamio, Takeyoshi Miki, Hirotada Mori, Eiichi Ohtsubo, Yoshiro Terawaki, Tetsuya Hayashi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.

Original languageEnglish
Pages (from-to)3194-3202
Number of pages9
JournalJournal of bacteriology
Volume184
Issue number12
DOIs
Publication statusPublished - Jun 8 2002
Externally publishedYes

Fingerprint

Plasmids
Genome
Open Reading Frames
Genes
Proteus vulgaris
Phenotype
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Complete nucleotide sequence of plasmid Rts1 : Implications for evolution of large plasmid genomes. / Murata, Takahiro; Ohnishi, Makoto; Ara, Takeshi; Kaneko, Jun; Han, Chang Gyun; Li, Yong Fang; Takashima, Kayoko; Nojima, Hideaki; Nakayama, Keisuke; Kaji, Akira; Kamio, Yoshiyuki; Miki, Takeyoshi; Mori, Hirotada; Ohtsubo, Eiichi; Terawaki, Yoshiro; Hayashi, Tetsuya.

In: Journal of bacteriology, Vol. 184, No. 12, 08.06.2002, p. 3194-3202.

Research output: Contribution to journalArticle

Murata, T, Ohnishi, M, Ara, T, Kaneko, J, Han, CG, Li, YF, Takashima, K, Nojima, H, Nakayama, K, Kaji, A, Kamio, Y, Miki, T, Mori, H, Ohtsubo, E, Terawaki, Y & Hayashi, T 2002, 'Complete nucleotide sequence of plasmid Rts1: Implications for evolution of large plasmid genomes', Journal of bacteriology, vol. 184, no. 12, pp. 3194-3202. https://doi.org/10.1128/JB.184.12.3194-3202.2002
Murata, Takahiro ; Ohnishi, Makoto ; Ara, Takeshi ; Kaneko, Jun ; Han, Chang Gyun ; Li, Yong Fang ; Takashima, Kayoko ; Nojima, Hideaki ; Nakayama, Keisuke ; Kaji, Akira ; Kamio, Yoshiyuki ; Miki, Takeyoshi ; Mori, Hirotada ; Ohtsubo, Eiichi ; Terawaki, Yoshiro ; Hayashi, Tetsuya. / Complete nucleotide sequence of plasmid Rts1 : Implications for evolution of large plasmid genomes. In: Journal of bacteriology. 2002 ; Vol. 184, No. 12. pp. 3194-3202.
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