Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain

Tian Qin, Hideki Hirakawa, Ken ichiro Iida, Kenshiro Oshima, Masahira Hattori, Kosuke Tashiro, Satoru Kuhara, Shin ichi Yoshida

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalPlasmid
Volume58
Issue number3
DOIs
Publication statusPublished - Nov 1 2007

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Legionella
Plasmids
Open Reading Frames
Proteins
DNA Polymerase II
DNA Polymerase III
Heavy Ions
DNA-Directed DNA Polymerase
Heavy Metals
Salmonella
Databases
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Qin, T., Hirakawa, H., Iida, K. I., Oshima, K., Hattori, M., Tashiro, K., ... Yoshida, S. I. (2007). Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain. Plasmid, 58(3), 261-268. https://doi.org/10.1016/j.plasmid.2007.08.001

Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain. / Qin, Tian; Hirakawa, Hideki; Iida, Ken ichiro; Oshima, Kenshiro; Hattori, Masahira; Tashiro, Kosuke; Kuhara, Satoru; Yoshida, Shin ichi.

In: Plasmid, Vol. 58, No. 3, 01.11.2007, p. 261-268.

Research output: Contribution to journalArticle

Qin, T, Hirakawa, H, Iida, KI, Oshima, K, Hattori, M, Tashiro, K, Kuhara, S & Yoshida, SI 2007, 'Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain', Plasmid, vol. 58, no. 3, pp. 261-268. https://doi.org/10.1016/j.plasmid.2007.08.001
Qin, Tian ; Hirakawa, Hideki ; Iida, Ken ichiro ; Oshima, Kenshiro ; Hattori, Masahira ; Tashiro, Kosuke ; Kuhara, Satoru ; Yoshida, Shin ichi. / Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain. In: Plasmid. 2007 ; Vol. 58, No. 3. pp. 261-268.
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