Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak

Katsushi Yokoyama, Kozo Makino, Yoshino Kubota, Motoji Watanabe, Sigenobu Kimura, Chikako H. Yutsudo, Ken Kurokawa, Kazuo Ishii, Masahira Hattori, Ichiro Tatsuno, Hiroyuki Abe, Myonsun Yoh, Tetsuya Iida, Makoto Ohnishi, Tetsuya Hayashi, Teruo Yasunaga, Takeshi Honda, Chihiro Sasakawa, Hideo Shinagawa

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Shiga toxins 1 and 2 (Stx1 and Stx2) are encoded by prophages lysogenized in enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains. Lytic growth of the phage particles carrying the stx1 genes (stx1A and stx1B) of the EHEC O157:H7 strain RIMD 0509952, which was derived from the Sakai outbreak in 1996 in Japan, was induced after treatment with mitomycin C, but the plaque formation of the phage was not detected. We have determined the complete nucleotide sequence of the prophage VT1-Sakai. The integration site of the prophage was identified within the yehV gene at 47.7min on the chromosome. The stx1 genes were downstream of the Q gene in the prophage genome, suggesting that their expression was regulated by the Q protein, the regulator of the late gene expression of the phage, which is similar to that of the stx1 or stx2 genes carried by the lambdoid phages reported previously. The sequences of the N gene and its recognition sites, nutL and nutR, were not homologous to those of the phages carrying the stx genes thus far reported, but they were very similar to those of bacteriophage φ21. The sequences of the repressor proteins, CI and Cro, that regulate expression of the early genes had low similarities with those of the known repressors of other phages, and their operator sequences were different from any sequence reported. These data suggest that multiple genetic recombination among bacteriophages with different immunities took place to generate the prophage VT1-Sakai. Comparison between the sequences of VT1-Sakai and lambda suggests that the ancestor of VT1-Sakai was produced by illegitimate excision, like lambda gal and bio phages. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)127-139
Number of pages13
JournalGene
Volume258
Issue number1-2
DOIs
Publication statusPublished - Nov 27 2000
Externally publishedYes

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Shiga Toxin 1
Enterohemorrhagic Escherichia coli
Prophages
Escherichia coli O157
Bacteriophages
Disease Outbreaks
Genes
Shiga Toxin 2
Lysogeny
vif Genes
Repressor Proteins
Gene Expression
Mitomycin
Regulator Genes
Genetic Recombination
Immunity
Japan
Chromosomes
Genome

All Science Journal Classification (ASJC) codes

  • Genetics

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Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak. / Yokoyama, Katsushi; Makino, Kozo; Kubota, Yoshino; Watanabe, Motoji; Kimura, Sigenobu; Yutsudo, Chikako H.; Kurokawa, Ken; Ishii, Kazuo; Hattori, Masahira; Tatsuno, Ichiro; Abe, Hiroyuki; Yoh, Myonsun; Iida, Tetsuya; Ohnishi, Makoto; Hayashi, Tetsuya; Yasunaga, Teruo; Honda, Takeshi; Sasakawa, Chihiro; Shinagawa, Hideo.

In: Gene, Vol. 258, No. 1-2, 27.11.2000, p. 127-139.

Research output: Contribution to journalArticle

Yokoyama, K, Makino, K, Kubota, Y, Watanabe, M, Kimura, S, Yutsudo, CH, Kurokawa, K, Ishii, K, Hattori, M, Tatsuno, I, Abe, H, Yoh, M, Iida, T, Ohnishi, M, Hayashi, T, Yasunaga, T, Honda, T, Sasakawa, C & Shinagawa, H 2000, 'Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak', Gene, vol. 258, no. 1-2, pp. 127-139. https://doi.org/10.1016/S0378-1119(00)00416-9
Yokoyama, Katsushi ; Makino, Kozo ; Kubota, Yoshino ; Watanabe, Motoji ; Kimura, Sigenobu ; Yutsudo, Chikako H. ; Kurokawa, Ken ; Ishii, Kazuo ; Hattori, Masahira ; Tatsuno, Ichiro ; Abe, Hiroyuki ; Yoh, Myonsun ; Iida, Tetsuya ; Ohnishi, Makoto ; Hayashi, Tetsuya ; Yasunaga, Teruo ; Honda, Takeshi ; Sasakawa, Chihiro ; Shinagawa, Hideo. / Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak. In: Gene. 2000 ; Vol. 258, No. 1-2. pp. 127-139.
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abstract = "Shiga toxins 1 and 2 (Stx1 and Stx2) are encoded by prophages lysogenized in enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains. Lytic growth of the phage particles carrying the stx1 genes (stx1A and stx1B) of the EHEC O157:H7 strain RIMD 0509952, which was derived from the Sakai outbreak in 1996 in Japan, was induced after treatment with mitomycin C, but the plaque formation of the phage was not detected. We have determined the complete nucleotide sequence of the prophage VT1-Sakai. The integration site of the prophage was identified within the yehV gene at 47.7min on the chromosome. The stx1 genes were downstream of the Q gene in the prophage genome, suggesting that their expression was regulated by the Q protein, the regulator of the late gene expression of the phage, which is similar to that of the stx1 or stx2 genes carried by the lambdoid phages reported previously. The sequences of the N gene and its recognition sites, nutL and nutR, were not homologous to those of the phages carrying the stx genes thus far reported, but they were very similar to those of bacteriophage φ21. The sequences of the repressor proteins, CI and Cro, that regulate expression of the early genes had low similarities with those of the known repressors of other phages, and their operator sequences were different from any sequence reported. These data suggest that multiple genetic recombination among bacteriophages with different immunities took place to generate the prophage VT1-Sakai. Comparison between the sequences of VT1-Sakai and lambda suggests that the ancestor of VT1-Sakai was produced by illegitimate excision, like lambda gal and bio phages. Copyright (C) 2000 Elsevier Science B.V.",
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T1 - Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak

AU - Yokoyama, Katsushi

AU - Makino, Kozo

AU - Kubota, Yoshino

AU - Watanabe, Motoji

AU - Kimura, Sigenobu

AU - Yutsudo, Chikako H.

AU - Kurokawa, Ken

AU - Ishii, Kazuo

AU - Hattori, Masahira

AU - Tatsuno, Ichiro

AU - Abe, Hiroyuki

AU - Yoh, Myonsun

AU - Iida, Tetsuya

AU - Ohnishi, Makoto

AU - Hayashi, Tetsuya

AU - Yasunaga, Teruo

AU - Honda, Takeshi

AU - Sasakawa, Chihiro

AU - Shinagawa, Hideo

PY - 2000/11/27

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N2 - Shiga toxins 1 and 2 (Stx1 and Stx2) are encoded by prophages lysogenized in enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains. Lytic growth of the phage particles carrying the stx1 genes (stx1A and stx1B) of the EHEC O157:H7 strain RIMD 0509952, which was derived from the Sakai outbreak in 1996 in Japan, was induced after treatment with mitomycin C, but the plaque formation of the phage was not detected. We have determined the complete nucleotide sequence of the prophage VT1-Sakai. The integration site of the prophage was identified within the yehV gene at 47.7min on the chromosome. The stx1 genes were downstream of the Q gene in the prophage genome, suggesting that their expression was regulated by the Q protein, the regulator of the late gene expression of the phage, which is similar to that of the stx1 or stx2 genes carried by the lambdoid phages reported previously. The sequences of the N gene and its recognition sites, nutL and nutR, were not homologous to those of the phages carrying the stx genes thus far reported, but they were very similar to those of bacteriophage φ21. The sequences of the repressor proteins, CI and Cro, that regulate expression of the early genes had low similarities with those of the known repressors of other phages, and their operator sequences were different from any sequence reported. These data suggest that multiple genetic recombination among bacteriophages with different immunities took place to generate the prophage VT1-Sakai. Comparison between the sequences of VT1-Sakai and lambda suggests that the ancestor of VT1-Sakai was produced by illegitimate excision, like lambda gal and bio phages. Copyright (C) 2000 Elsevier Science B.V.

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