Complete sequence-specific 1H nuclear magnetic resonance assignments for mouse epidermal growth factor

Daisuke Kohda, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The 1H NMR spectrum of the mouse epidermal growth factor (53 residues) was analyzed with the use of two-dimensional NMR techniques. All the observable 296 proton resonances were completely assigned in a sequential manner. For the spin system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was useful, especially for arginine and proline residues. The easy spin system identification of these long-side-chainbearing amino acid residues greatly facilitated the sequence-specific resonance assignment of the epidermal growth factor.

Original languageEnglish
Pages (from-to)554-571
Number of pages18
JournalJournal of biochemistry
Volume103
Issue number3
DOIs
Publication statusPublished - Jan 1 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Complete sequence-specific <sup>1</sup>H nuclear magnetic resonance assignments for mouse epidermal growth factor'. Together they form a unique fingerprint.

  • Cite this