Complete sequence-specific 1H nuclear magnetic resonance assignments for mouse epidermal growth factor

Daisuke Kohda, Fuyuhiko Inagaki

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


The 1H NMR spectrum of the mouse epidermal growth factor (53 residues) was analyzed with the use of two-dimensional NMR techniques. All the observable 296 proton resonances were completely assigned in a sequential manner. For the spin system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was useful, especially for arginine and proline residues. The easy spin system identification of these long-side-chainbearing amino acid residues greatly facilitated the sequence-specific resonance assignment of the epidermal growth factor.

Original languageEnglish
Pages (from-to)554-571
Number of pages18
JournalJournal of biochemistry
Issue number3
Publication statusPublished - Jan 1 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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