The 1H NMR spectrum of the mouse epidermal growth factor (53 residues) was analyzed with the use of two-dimensional NMR techniques. All the observable 296 proton resonances were completely assigned in a sequential manner. For the spin system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was useful, especially for arginine and proline residues. The easy spin system identification of these long-side-chainbearing amino acid residues greatly facilitated the sequence-specific resonance assignment of the epidermal growth factor.
|Number of pages||18|
|Journal||Journal of biochemistry|
|Publication status||Published - Jan 1 1988|
All Science Journal Classification (ASJC) codes
- Molecular Biology