Complex formation of cytochrome c with a calixarene carboxylic acid derivative: A novel solubilization method for biomolecules in organic media

Tatsuya Oshima, Masahiro Goto, Shintaro Furusaki

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A calixarene carboxylic acid derivative has been found to form a complex with the cationic protein cytochrome c. The solubilized cytochrome c was stable and showed peroxidase activity in chloroform. The calix[6]arene and the calix[8]arene achieved quantitative extraction of the protein. The calix[6]arene, whose cavity is well-fitted to a protonated amino group, exhibited a selectivity to lysine-rich proteins due to the recognition of the ε-amino groups in lysine residues on the surface of the protein. This is the first report showing protein extraction by calixarenes. The solubilized cytochrome c could catalyze an oxidative reaction in organic solvents. This host compound functions as a novel solubilization tool for biomolecules and a separation tool for lysine-rich proteins.

Original languageEnglish
Pages (from-to)438-444
Number of pages7
JournalBiomacromolecules
Volume3
Issue number3
DOIs
Publication statusPublished - Jun 8 2002

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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