Complex formation of cytochrome c with a calixarene carboxylic acid derivative: A novel solubilization method for biomolecules in organic media

Tatsuya Oshima, Masahiro Goto, Shintaro Furusaki

    Research output: Contribution to journalArticlepeer-review

    61 Citations (Scopus)

    Abstract

    A calixarene carboxylic acid derivative has been found to form a complex with the cationic protein cytochrome c. The solubilized cytochrome c was stable and showed peroxidase activity in chloroform. The calix[6]arene and the calix[8]arene achieved quantitative extraction of the protein. The calix[6]arene, whose cavity is well-fitted to a protonated amino group, exhibited a selectivity to lysine-rich proteins due to the recognition of the ε-amino groups in lysine residues on the surface of the protein. This is the first report showing protein extraction by calixarenes. The solubilized cytochrome c could catalyze an oxidative reaction in organic solvents. This host compound functions as a novel solubilization tool for biomolecules and a separation tool for lysine-rich proteins.

    Original languageEnglish
    Pages (from-to)438-444
    Number of pages7
    JournalBiomacromolecules
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - 2002

    All Science Journal Classification (ASJC) codes

    • Bioengineering
    • Biomaterials
    • Polymers and Plastics
    • Materials Chemistry

    Fingerprint Dive into the research topics of 'Complex formation of cytochrome c with a calixarene carboxylic acid derivative: A novel solubilization method for biomolecules in organic media'. Together they form a unique fingerprint.

    Cite this