Composition and positional distribution of fatty acids in polar lipids from Chlorella ellipsoidea differing in chilling susceptibility and frost hardiness

Toshio Joh, Tsuguhiko Yoshida, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The composition and positional distribution of fatty acids in the polar lipids from 4 strains of Chlorella differing in chilling susceptibility and frost hardiness were analyzed by enzymatic hydrolysis and gas‐liquid chromatography. Analysis of the polar lipids from chilling‐sensitive, chilling‐resistant and chilling‐sensitive revertant strains of Chlorella ellipsoidea IAM C‐102 showed that the sum of palmitic and trans‐3‐hexadecenoic acid in phosphatidylglycerol (PG) is about 60% for the sensitive strains and 53% for the resistant strain. The sum of dipalmitoyl and 1‐palmitoyl‐2‐(trans‐3‐hexadecenoyl) PG as estimated from the positional distribution of their fatty acids, is about 10% in the case of each of the three strains. The contents of unsaturated fatty acids in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were higher in the resistant than in the sensitive strain. This suggests that unsaturation of fatty acids in not only PG but also PC and PE is involved in chilling sensitivity of Chlorella. On the other hand, lipid changes during the development of frost hardiness of C. ellipsoidea IAM C‐27, a frost hardy strain, were examined. The results showed that fatty acids in most lipid classes are unsaturated in the hardening process but their degree of unsaturation is not greatly different from that of the chilling‐resistant strain, suggesting that not only unsaturation of fatty acids in lipids but also other factors are necessary for the development of frost hardiness.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalPhysiologia Plantarum
Volume89
Issue number2
DOIs
Publication statusPublished - Jan 1 1993

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Chlorella ellipsoidea
Chlorella
frost
Fatty Acids
Phosphatidylglycerols
fatty acids
Lipids
lipids
phosphatidylglycerols
Phosphatidylcholines
phosphatidylethanolamines
phosphatidylcholines
Palmitic Acid
Unsaturated Fatty Acids
Chromatography
Hydrolysis
cold treatment
enzymatic hydrolysis
unsaturated fatty acids
chromatography

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Composition and positional distribution of fatty acids in polar lipids from Chlorella ellipsoidea differing in chilling susceptibility and frost hardiness. / Joh, Toshio; Yoshida, Tsuguhiko; Yoshimoto, Makoto; Miyamoto, Takahisa; Hatano, Shoji.

In: Physiologia Plantarum, Vol. 89, No. 2, 01.01.1993, p. 285-290.

Research output: Contribution to journalArticle

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