Composition of culture medium is more important than co-culture with hepatic non-parenchymal cells in albumin production activity of primary rat hepatocytes, and the effect was enhanced by hepatocytes spheroid culture in collagen gel

Hiroyuki Ijima, Yasuo Kakeya, Toru Yokonuma, Yung Te Hou, Takayuki Takei

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Co-culture of primary rat hepatocytes with hepatic non-parenchymal cells or sinusoidal endothelial cells for albumin production activity as an index of liver-specific function was studied. The co-cultures were effective for the expression and maintenance of albumin production activity. However, the co-culture effect was not observed when we used a suitable culture medium, which had already been reported to be sufficient for albumin production activity. Albumin production of dispersed cells in collagen gel culture was higher than that of spheroid culture. In addition, albumin production of spheroids in collagen gel culture was higher than that of spheroid culture and dispersed cell collagen gel culture with a suitable culture medium. We found that culture medium composition was more important than co-culture for expression and maintenance of albumin production. Furthermore, we found that cell-cell interaction was effective for the expression of albumin production, but heterotypic cell-cell interaction was not necessary.

Original languageEnglish
Pages (from-to)226-231
Number of pages6
JournalBiochemical Engineering Journal
Volume45
Issue number3
DOIs
Publication statusPublished - Aug 15 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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