Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism

Tomonori Shinya, Ivan Gális, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

Original languageEnglish
Pages (from-to)1404-1413
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number10
DOIs
Publication statusPublished - Oct 1 2007

Fingerprint

Glucans
phenylpropanoids
glucans
Tobacco
tobacco
Transcription Factors
transcription factors
Alternaria alternata
Gene Expression
gene expression
metabolism
Genes
Alternaria
genes
cells
Microarray Analysis
global change
Oligonucleotide Array Sequence Analysis
Fungi
fungi

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism. / Shinya, Tomonori; Gális, Ivan; Narisawa, Tomoko; Sasaki, Mami; Fukuda, Hiroo; Matsuoka, Hideaki; Saito, Mikako; Matsuoka, Ken.

In: Plant and Cell Physiology, Vol. 48, No. 10, 01.10.2007, p. 1404-1413.

Research output: Contribution to journalArticle

Shinya, Tomonori ; Gális, Ivan ; Narisawa, Tomoko ; Sasaki, Mami ; Fukuda, Hiroo ; Matsuoka, Hideaki ; Saito, Mikako ; Matsuoka, Ken. / Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 10. pp. 1404-1413.
@article{c8f6e96076984c3f84b3f20b9db0c123,
title = "Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism",
abstract = "We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.",
author = "Tomonori Shinya and Ivan G{\'a}lis and Tomoko Narisawa and Mami Sasaki and Hiroo Fukuda and Hideaki Matsuoka and Mikako Saito and Ken Matsuoka",
year = "2007",
month = "10",
day = "1",
doi = "10.1093/pcp/pcm115",
language = "English",
volume = "48",
pages = "1404--1413",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism

AU - Shinya, Tomonori

AU - Gális, Ivan

AU - Narisawa, Tomoko

AU - Sasaki, Mami

AU - Fukuda, Hiroo

AU - Matsuoka, Hideaki

AU - Saito, Mikako

AU - Matsuoka, Ken

PY - 2007/10/1

Y1 - 2007/10/1

N2 - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

AB - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

UR - http://www.scopus.com/inward/record.url?scp=35348901404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35348901404&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcm115

DO - 10.1093/pcp/pcm115

M3 - Article

C2 - 17761750

AN - SCOPUS:35348901404

VL - 48

SP - 1404

EP - 1413

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 10

ER -