Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases

Takeru Yoshimura, Kohei Sonoda, Mika Sugahara, Yasutaka Mochizuki, Hiroshi Enaida, Yuji Oshima, Akifumi Ueno, Yasuaki Hata, Hiroki Yoshida, Tatsuro Ishibashi

Research output: Contribution to journalArticle

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Abstract

Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a total of 83 patients with either idiopathic macular hole (MH) or idiopathic epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, such as ischemic retina and proliferative membranes. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with control group. According to the correlation analysis in the individual patient's level, these three factors that were simultaneously increased, did not show any independent upregulation in all the examined diseases. Vascular endothelial growth factor (VEGF) was significantly elevated in patients with PDR and CRVO. In PDR patients, the elevation of VEGF was significantly correlated with the three factors: IL-6, IL-8, and MCP-1, while no significant correlation was observed in CRVO patients. In conclusion, multiplex bead system enabled a comprehensive soluble factor analysi in vitreous fluid derived from variety of patients. Major three factors: IL-6, IL-8, and MCP-1 were strongly correlated with each other indicating a common pathway involved in inflammation process in vitreoretinal diseases.

Original languageEnglish
Article numbere8158
JournalPLoS One
Volume4
Issue number12
DOIs
Publication statusPublished - Dec 1 2009

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Chemokine CCL2
Interleukin-8
Interleukin-6
interleukin-8
Vascular Endothelial Growth Factor A
interleukin-6
Retinal Vein Occlusion
vascular endothelial growth factors
Membranes
Fluids
Chemokines
inflammation
Intercellular Signaling Peptides and Proteins
diabetic retinopathy
Epiretinal Membrane
Inflammation
Retinal Vein
Retinal Perforations
Cytokines
Macular Edema

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases. / Yoshimura, Takeru; Sonoda, Kohei; Sugahara, Mika; Mochizuki, Yasutaka; Enaida, Hiroshi; Oshima, Yuji; Ueno, Akifumi; Hata, Yasuaki; Yoshida, Hiroki; Ishibashi, Tatsuro.

In: PLoS One, Vol. 4, No. 12, e8158, 01.12.2009.

Research output: Contribution to journalArticle

Yoshimura, T, Sonoda, K, Sugahara, M, Mochizuki, Y, Enaida, H, Oshima, Y, Ueno, A, Hata, Y, Yoshida, H & Ishibashi, T 2009, 'Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases', PLoS One, vol. 4, no. 12, e8158. https://doi.org/10.1371/journal.pone.0008158
Yoshimura, Takeru ; Sonoda, Kohei ; Sugahara, Mika ; Mochizuki, Yasutaka ; Enaida, Hiroshi ; Oshima, Yuji ; Ueno, Akifumi ; Hata, Yasuaki ; Yoshida, Hiroki ; Ishibashi, Tatsuro. / Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases. In: PLoS One. 2009 ; Vol. 4, No. 12.
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