Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray

Yoshiaki Kouzuma, Yusuke Tsutsumi, Masumi Abe, Takeshi Hayashi, Kazumasa Hada, Keigo Uehashi, Yukiko Shimada, Kosuke Tashiro, Satoru Kuhara, Makoto Kimura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Wound-inducible gene expression in the the Nicotiana glutinosa leaves was studied by using a microarray with 9600 full-length cDNAs. As a result, 86 genes were identified as wound-inducible genes in the N. glutinosa leaves, including those encoding defense related proteins, such as heat shock proteins, glutathione S-transferase, ascorbate peroxidase and non-specific lipid-transfer proteins. Among 86 genes, 15 genes including 11 hypothetical protein genes and 1 unknown protein genes encode unknown functional proteins. Although the translational products of these genes have not been characterized, they are potential candidates for defense-related proteins toward wounding. The cluster analysis classified the genes into 6 groups on the basis of their expression patterns. It is likely that genes clustered in the same groups may be co-regulated by common transcriptional factors and also translational products belonging to the same clusters may share common functions in defense response to wounding.

Original languageEnglish
Pages (from-to)635-648
Number of pages14
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number2
Publication statusPublished - Oct 1 2005

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Nicotiana glutinosa
plant damage
Oligonucleotide Array Sequence Analysis
Tobacco
Wounds and Injuries
Genes
leaves
genes
Proteins
proteins
Ascorbate Peroxidases
Protein S
Heat-Shock Proteins
Glutathione Transferase
Cluster Analysis
ascorbate peroxidase
Complementary DNA
heat shock proteins
glutathione transferase
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray. / Kouzuma, Yoshiaki; Tsutsumi, Yusuke; Abe, Masumi; Hayashi, Takeshi; Hada, Kazumasa; Uehashi, Keigo; Shimada, Yukiko; Tashiro, Kosuke; Kuhara, Satoru; Kimura, Makoto.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 2, 01.10.2005, p. 635-648.

Research output: Contribution to journalArticle

Kouzuma, Y, Tsutsumi, Y, Abe, M, Hayashi, T, Hada, K, Uehashi, K, Shimada, Y, Tashiro, K, Kuhara, S & Kimura, M 2005, 'Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray', Journal of the Faculty of Agriculture, Kyushu University, vol. 50, no. 2, pp. 635-648.
Kouzuma, Yoshiaki ; Tsutsumi, Yusuke ; Abe, Masumi ; Hayashi, Takeshi ; Hada, Kazumasa ; Uehashi, Keigo ; Shimada, Yukiko ; Tashiro, Kosuke ; Kuhara, Satoru ; Kimura, Makoto. / Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray. In: Journal of the Faculty of Agriculture, Kyushu University. 2005 ; Vol. 50, No. 2. pp. 635-648.
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