Abstract
Wound-inducible gene expression in the the Nicotiana glutinosa leaves was studied by using a microarray with 9600 full-length cDNAs. As a result, 86 genes were identified as wound-inducible genes in the N. glutinosa leaves, including those encoding defense related proteins, such as heat shock proteins, glutathione S-transferase, ascorbate peroxidase and non-specific lipid-transfer proteins. Among 86 genes, 15 genes including 11 hypothetical protein genes and 1 unknown protein genes encode unknown functional proteins. Although the translational products of these genes have not been characterized, they are potential candidates for defense-related proteins toward wounding. The cluster analysis classified the genes into 6 groups on the basis of their expression patterns. It is likely that genes clustered in the same groups may be co-regulated by common transcriptional factors and also translational products belonging to the same clusters may share common functions in defense response to wounding.
| Original language | English |
|---|---|
| Pages (from-to) | 635-648 |
| Number of pages | 14 |
| Journal | Journal of the Faculty of Agriculture, Kyushu University |
| Volume | 50 |
| Issue number | 2 |
| Publication status | Published - Oct 1 2005 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
Cite this
Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray. / Kouzuma, Yoshiaki; Tsutsumi, Yusuke; Abe, Masumi; Hayashi, Takeshi; Hada, Kazumasa; Uehashi, Keigo; Shimada, Yukiko; Tashiro, Kosuke; Kuhara, Satoru; Kimura, Makoto.
In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 2, 01.10.2005, p. 635-648.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray
AU - Kouzuma, Yoshiaki
AU - Tsutsumi, Yusuke
AU - Abe, Masumi
AU - Hayashi, Takeshi
AU - Hada, Kazumasa
AU - Uehashi, Keigo
AU - Shimada, Yukiko
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Kimura, Makoto
PY - 2005/10/1
Y1 - 2005/10/1
N2 - Wound-inducible gene expression in the the Nicotiana glutinosa leaves was studied by using a microarray with 9600 full-length cDNAs. As a result, 86 genes were identified as wound-inducible genes in the N. glutinosa leaves, including those encoding defense related proteins, such as heat shock proteins, glutathione S-transferase, ascorbate peroxidase and non-specific lipid-transfer proteins. Among 86 genes, 15 genes including 11 hypothetical protein genes and 1 unknown protein genes encode unknown functional proteins. Although the translational products of these genes have not been characterized, they are potential candidates for defense-related proteins toward wounding. The cluster analysis classified the genes into 6 groups on the basis of their expression patterns. It is likely that genes clustered in the same groups may be co-regulated by common transcriptional factors and also translational products belonging to the same clusters may share common functions in defense response to wounding.
AB - Wound-inducible gene expression in the the Nicotiana glutinosa leaves was studied by using a microarray with 9600 full-length cDNAs. As a result, 86 genes were identified as wound-inducible genes in the N. glutinosa leaves, including those encoding defense related proteins, such as heat shock proteins, glutathione S-transferase, ascorbate peroxidase and non-specific lipid-transfer proteins. Among 86 genes, 15 genes including 11 hypothetical protein genes and 1 unknown protein genes encode unknown functional proteins. Although the translational products of these genes have not been characterized, they are potential candidates for defense-related proteins toward wounding. The cluster analysis classified the genes into 6 groups on the basis of their expression patterns. It is likely that genes clustered in the same groups may be co-regulated by common transcriptional factors and also translational products belonging to the same clusters may share common functions in defense response to wounding.
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M3 - Article
AN - SCOPUS:27744487245
VL - 50
SP - 635
EP - 648
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 2
ER -