Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray

Yoshiaki Kouzuma, Yusuke Tsutsumi, Masumi Abe, Takeshi Hayashi, Kazumasa Hada, Keigo Uehashi, Yukiko Shimada, Kosuke Tashiro, Satoru Kuhara, Makoto Kimura

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1 Citation (Scopus)

Abstract

Wound-inducible gene expression in the the Nicotiana glutinosa leaves was studied by using a microarray with 9600 full-length cDNAs. As a result, 86 genes were identified as wound-inducible genes in the N. glutinosa leaves, including those encoding defense related proteins, such as heat shock proteins, glutathione S-transferase, ascorbate peroxidase and non-specific lipid-transfer proteins. Among 86 genes, 15 genes including 11 hypothetical protein genes and 1 unknown protein genes encode unknown functional proteins. Although the translational products of these genes have not been characterized, they are potential candidates for defense-related proteins toward wounding. The cluster analysis classified the genes into 6 groups on the basis of their expression patterns. It is likely that genes clustered in the same groups may be co-regulated by common transcriptional factors and also translational products belonging to the same clusters may share common functions in defense response to wounding.

Original languageEnglish
Pages (from-to)635-648
Number of pages14
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number2
Publication statusPublished - Oct 2005

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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    Kouzuma, Y., Tsutsumi, Y., Abe, M., Hayashi, T., Hada, K., Uehashi, K., Shimada, Y., Tashiro, K., Kuhara, S., & Kimura, M. (2005). Comprehensive analysis of wound-inducible genes from the Nicotiana glutinosa leaves using a full-length cDNA microarray. Journal of the Faculty of Agriculture, Kyushu University, 50(2), 635-648.