Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus

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Abstract

DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.

Original languageEnglish
Pages (from-to)681-691
Number of pages11
JournalNucleosides, Nucleotides and Nucleic Acids
Volume25
Issue number4-6
DOIs
Publication statusPublished - Jun 1 2006

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Pyrococcus furiosus
Archaea
DNA-Directed DNA Polymerase
DNA
Clamping devices
Proliferating Cell Nuclear Antigen
Cell Extracts
DNA Replication
Western Blotting

All Science Journal Classification (ASJC) codes

  • Genetics
  • Biochemistry

Cite this

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