Abstract
DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 681-691 |
| Number of pages | 11 |
| Journal | Nucleosides, Nucleotides and Nucleic Acids |
| Volume | 25 |
| Issue number | 4-6 |
| DOIs | |
| Publication status | Published - Jun 1 2006 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Biochemistry
Cite this
Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus. / Ishino, Sonoko; Ishino, Yoshizumi.
In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 25, No. 4-6, 01.06.2006, p. 681-691.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus
AU - Ishino, Sonoko
AU - Ishino, Yoshizumi
PY - 2006/6/1
Y1 - 2006/6/1
N2 - DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.
AB - DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=33747617313&partnerID=8YFLogxK
U2 - 10.1080/15257770600686485
DO - 10.1080/15257770600686485
M3 - Article
C2 - 16838855
AN - SCOPUS:33747617313
VL - 25
SP - 681
EP - 691
JO - Nucleosides and Nucleotides
JF - Nucleosides and Nucleotides
SN - 0732-8311
IS - 4-6
ER -