Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus

Sonoko Ishino; Yoshizumi Ishino

doi:10.1080/15257770600686485

Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus

Sonoko Ishino, Yoshizumi Ishino

Molecular Biosciences

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.

Original language	English
Pages (from-to)	681-691
Number of pages	11
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	25
Issue number	4-6
DOIs	https://doi.org/10.1080/15257770600686485
Publication status	Published - Jun 1 2006

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Genetics

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10.1080/15257770600686485

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