Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging

Masakazu Umezawa, Toshihiro Sera, Hideo Yokota, Maho Takematsu, Masahiko Morita, Gil Yeroslavsky, Masao Kamimura, Kohei Soga

Research output: Contribution to journalArticle

Abstract

This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.

Original languageEnglish
Article numbere202000071
JournalJournal of Biophotonics
Volume13
Issue number8
DOIs
Publication statusPublished - Aug 1 2020

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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    Umezawa, M., Sera, T., Yokota, H., Takematsu, M., Morita, M., Yeroslavsky, G., Kamimura, M., & Soga, K. (2020). Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging. Journal of Biophotonics, 13(8), [e202000071]. https://doi.org/10.1002/jbio.202000071