Concentrate mineralogy dictates the composition of bioleaching microbial consortia

D. Barrie Johnson, Liu Yajie, Naoko Okibe, Kris Coupland, Kevin B. Hallberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Twelve mineral concentrates and one polymetallic ore were subjected to bioleaching by a heterogeneous mixed culture of moderately thermophilic prokaryotes in shake flask cultures incubated at 45°C. Dissolution of minerals was monitored, and the compositions of the consortia that developed in each case were determined using a combined cultivation-dependent (plating on selective solid media) and biomolecular (terminal restriction fragment length polymorphism) approach [1]. The concentrates used were divided into: (i) pyrite concentrates (two gold- and one cobalt-enriched); (ii) chalcopyrite (three concentrates, containing between 16 and 1500 g/t silver); (iii) copper-nickel (two concentrates); (iv) single concentrates of enargite; zinc (sphalerite) and lead (galena) and a copper-rich (predominantly chalcocite) black shale. The single ore sample (a polymetallic black schist) contained 0.3% nickel, 0.2% copper and 0.6% zinc. The cultures were inoculated with a physiologically and phylogenetically diverse range of moderately thermophilic acidophiles [2], comprising ten bacteria and one archaeon. These were: (i) iron- or sulfur-oxidizing autotrophs {Leptospirillum ferriphilum and At. caldus); (ii) iron-oxidizing mixotrophs/heterotrophs (Acidimicrobium ferrooxidans, a Ferroplasma sp. and "Ferrithrix thermotolerans" Y005); (iii) iron- and sulfur-oxidizing mixotrophs (four Firmicutes, including three Sulfobacillus spp. and one unclassified species); (iv) iron-reducing heterotrophic bacteria (Acidicaldus organivorans and Alicyclobacillus Y004). The bacteria and archaea were grown in appropriate liquid media [1] and inoculated into sterile mineral cultures in similar numbers. With the single exception of the enargite concentrate, all mineral samples were leached successfully by the moderately thermophilic consortium, often with >80% of the target metal(s) solubilised within 30 days. The compositions of the microbial communities that developed from the initial inocula were, however, highly variable (Table 1). In most cases, the established communities included both iron-and sulfur-oxidisers, and also both autotrophic and heterotrophic acidophiles. Notable exceptions were the chalcopyrite concentrates which were dominated (exclusively so in one case) by the mixotroph Sb. thermosulfidooxidans. Am. ferrooxidans was also frequently found to be an important (in terms of relative abundance) member of the effective bioleaching consortia, which is interesting as this iro-noxidiser has rarely been reported in bioleaching operations.

Original languageEnglish
Title of host publicationBiohydrometallurgy
Subtitle of host publicationFrom the Single Cell to the Environment
Pages403-404
Number of pages2
Publication statusPublished - Dec 1 2007
Event17th International Biohydrometallurgy Symposium, IBS 2007 - Frankfurt am Main, Germany
Duration: Sep 2 2007Sep 5 2007

Publication series

NameAdvanced Materials Research
Volume20-21
ISSN (Print)1022-6680

Other

Other17th International Biohydrometallurgy Symposium, IBS 2007
CountryGermany
CityFrankfurt am Main
Period9/2/079/5/07

Fingerprint

Bioleaching
Mineralogy
Iron
Minerals
Chemical analysis
Bacteria
Sulfur
Copper
Ores
Zinc
Nickel
Pyrites
Shale
Polymorphism
Plating
Cobalt
Dissolution
Silver
Lead
Gold

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Johnson, D. B., Yajie, L., Okibe, N., Coupland, K., & Hallberg, K. B. (2007). Concentrate mineralogy dictates the composition of bioleaching microbial consortia. In Biohydrometallurgy: From the Single Cell to the Environment (pp. 403-404). (Advanced Materials Research; Vol. 20-21).

Concentrate mineralogy dictates the composition of bioleaching microbial consortia. / Johnson, D. Barrie; Yajie, Liu; Okibe, Naoko; Coupland, Kris; Hallberg, Kevin B.

Biohydrometallurgy: From the Single Cell to the Environment. 2007. p. 403-404 (Advanced Materials Research; Vol. 20-21).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Johnson, DB, Yajie, L, Okibe, N, Coupland, K & Hallberg, KB 2007, Concentrate mineralogy dictates the composition of bioleaching microbial consortia. in Biohydrometallurgy: From the Single Cell to the Environment. Advanced Materials Research, vol. 20-21, pp. 403-404, 17th International Biohydrometallurgy Symposium, IBS 2007, Frankfurt am Main, Germany, 9/2/07.
Johnson DB, Yajie L, Okibe N, Coupland K, Hallberg KB. Concentrate mineralogy dictates the composition of bioleaching microbial consortia. In Biohydrometallurgy: From the Single Cell to the Environment. 2007. p. 403-404. (Advanced Materials Research).
Johnson, D. Barrie ; Yajie, Liu ; Okibe, Naoko ; Coupland, Kris ; Hallberg, Kevin B. / Concentrate mineralogy dictates the composition of bioleaching microbial consortia. Biohydrometallurgy: From the Single Cell to the Environment. 2007. pp. 403-404 (Advanced Materials Research).
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abstract = "Twelve mineral concentrates and one polymetallic ore were subjected to bioleaching by a heterogeneous mixed culture of moderately thermophilic prokaryotes in shake flask cultures incubated at 45°C. Dissolution of minerals was monitored, and the compositions of the consortia that developed in each case were determined using a combined cultivation-dependent (plating on selective solid media) and biomolecular (terminal restriction fragment length polymorphism) approach [1]. The concentrates used were divided into: (i) pyrite concentrates (two gold- and one cobalt-enriched); (ii) chalcopyrite (three concentrates, containing between 16 and 1500 g/t silver); (iii) copper-nickel (two concentrates); (iv) single concentrates of enargite; zinc (sphalerite) and lead (galena) and a copper-rich (predominantly chalcocite) black shale. The single ore sample (a polymetallic black schist) contained 0.3{\%} nickel, 0.2{\%} copper and 0.6{\%} zinc. The cultures were inoculated with a physiologically and phylogenetically diverse range of moderately thermophilic acidophiles [2], comprising ten bacteria and one archaeon. These were: (i) iron- or sulfur-oxidizing autotrophs {Leptospirillum ferriphilum and At. caldus); (ii) iron-oxidizing mixotrophs/heterotrophs (Acidimicrobium ferrooxidans, a Ferroplasma sp. and {"}Ferrithrix thermotolerans{"} Y005); (iii) iron- and sulfur-oxidizing mixotrophs (four Firmicutes, including three Sulfobacillus spp. and one unclassified species); (iv) iron-reducing heterotrophic bacteria (Acidicaldus organivorans and Alicyclobacillus Y004). The bacteria and archaea were grown in appropriate liquid media [1] and inoculated into sterile mineral cultures in similar numbers. With the single exception of the enargite concentrate, all mineral samples were leached successfully by the moderately thermophilic consortium, often with >80{\%} of the target metal(s) solubilised within 30 days. The compositions of the microbial communities that developed from the initial inocula were, however, highly variable (Table 1). In most cases, the established communities included both iron-and sulfur-oxidisers, and also both autotrophic and heterotrophic acidophiles. Notable exceptions were the chalcopyrite concentrates which were dominated (exclusively so in one case) by the mixotroph Sb. thermosulfidooxidans. Am. ferrooxidans was also frequently found to be an important (in terms of relative abundance) member of the effective bioleaching consortia, which is interesting as this iro-noxidiser has rarely been reported in bioleaching operations.",
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