Concentration of bacteria in high conductive medium using negative dielectrophoresis

Yuki Inoue, Ryoji Obara, Michihiko Nakano, Junya Suehiro

Research output: Contribution to conferencePaper

3 Citations (Scopus)

Abstract

Rapid and sensitive detection of pathogenic bacteria has been an important concern in various fields such as bioscience research, medical diagnosis and hazard analysis in the food industry. The requirement for highly efficient bacterial detection has pointed to the need for new and innovative sample concentration technologies. In this study, a new microfluidic device was demonstrated to enable concentration of bacteria by using negative dielectrophoresis (n-DEP) in high conductive medium. A special design of the electrode system, which was covered with patterned dielectric films, was tested in order to control the electric field distribution in the device so that n-DEP force could drive cells to a point for concentration as well as Joule heating of the suspension medium could be suppressed to avoid heat-induced cell damage. The electrode design was optimized using numerical calculation results of cell motion trajectories and the suspension medium temperature in the device. It was experimentally proved that proposed microfluidic device could concentrate E. coli cells in a continuous flow of cell suspension.

Original languageEnglish
Pages3336-3340
Number of pages5
DOIs
Publication statusPublished - Jun 16 2015
Event2015 IEEE International Conference on Industrial Technology, ICIT 2015 - Seville, Spain
Duration: Mar 17 2015Mar 19 2015

Other

Other2015 IEEE International Conference on Industrial Technology, ICIT 2015
CountrySpain
CitySeville
Period3/17/153/19/15

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All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Inoue, Y., Obara, R., Nakano, M., & Suehiro, J. (2015). Concentration of bacteria in high conductive medium using negative dielectrophoresis. 3336-3340. Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain. https://doi.org/10.1109/ICIT.2015.7125592