Conditions required for a hybrid artificial liver support system using a PUF/hepatocyte-spheroid packed-bed module and its use in dogs with liver failure

Hiroyuki Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, K. Funatsu

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20 Citations (Scopus)


We studied the effects of a hybrid artificial liver support system we developed on dogs with hepatic failure. The system consisted of a multi-channel polyurethane foam packed-bed culture module, including primary dog hepatocyte spheroids. Blood ammonia was well metabolized by 20 g hepatocytes, but the other functions such as glucose concentration, total bile acid concentration, and survival time required 30 g hepatocytes to improve conditions. We found that we should use a culture substratum that easily forms spheroids, and that an artificial liver module should be used as soon as possible after spheroid formation by hepatocytes in the module.

Original languageEnglish
Pages (from-to)446-453
Number of pages8
JournalInternational Journal of Artificial Organs
Issue number7
Publication statusPublished - Aug 31 2000


All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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