We studied the effects of a hybrid artificial liver support system we developed on dogs with hepatic failure. The system consisted of a multi-channel polyurethane foam packed-bed culture module, including primary dog hepatocyte spheroids. Blood ammonia was well metabolized by 20 g hepatocytes, but the other functions such as glucose concentration, total bile acid concentration, and survival time required 30 g hepatocytes to improve conditions. We found that we should use a culture substratum that easily forms spheroids, and that an artificial liver module should be used as soon as possible after spheroid formation by hepatocytes in the module.
|Number of pages||8|
|Journal||International Journal of Artificial Organs|
|Publication status||Published - Aug 31 2000|
All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Biomedical Engineering