Confirmation of in vitro synthesis of 2-deoxy-sc7llo-inosose, the earliest intermediate in the biosynthesis of 2-deoxystreptamine, using cell free preparations of streptomyces fradiae

Noriaki Yamauchi, Katsumi Kakinuma

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The in vitro activity of formation of 2-deoxy-scyllo-inosose, the earliest intermediate of the 2-deoxystreptamine biosynthesis, was confirmed in the precipitate fraction obtained by 30~45% saturation of ammonium sulfate from the 15,000 × g supernatant of the sonicated Streptomyces fradiae IFO 13147 cells.

Original languageEnglish
Pages (from-to)774-780
Number of pages7
JournalJournal of Antibiotics
Volume45
Issue number5
DOIs
Publication statusPublished - 1992
Externally publishedYes

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Ammonium Sulfate
Streptomyces
2-deoxyinosose
inosose
2-deoxystreptamine
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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title = "Confirmation of in vitro synthesis of 2-deoxy-sc7llo-inosose, the earliest intermediate in the biosynthesis of 2-deoxystreptamine, using cell free preparations of streptomyces fradiae",
abstract = "The in vitro activity of formation of 2-deoxy-scyllo-inosose, the earliest intermediate of the 2-deoxystreptamine biosynthesis, was confirmed in the precipitate fraction obtained by 30~45{\%} saturation of ammonium sulfate from the 15,000 × g supernatant of the sonicated Streptomyces fradiae IFO 13147 cells.",
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AB - The in vitro activity of formation of 2-deoxy-scyllo-inosose, the earliest intermediate of the 2-deoxystreptamine biosynthesis, was confirmed in the precipitate fraction obtained by 30~45% saturation of ammonium sulfate from the 15,000 × g supernatant of the sonicated Streptomyces fradiae IFO 13147 cells.

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