Conformation and position of membrane-bound amphotericin B deduced from NMR in SDS micelles

Nobuaki Matsumori, Toshihiro Houdai, Michio Murata

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

(Figure Presented) Amphotericin B (AmB) is known to self-assemble to form an ion channel across lipid bilayer membranes. To gain insight into the conformation of AmB in lipidic environments, AmB in SDS micelles was subjected to high-resolution NMR and CD measurements, and the NMR-derived conformation thus obtained was refined by molecular mechanics calculations. These results indicate that AmB in SDS micelles is conformationally fixed particularly for the macrolide moiety. Paramagnetic relaxation experiments with the use of Mn 2+ reveal that AmB is shallowly embedded in the micelle with the polyhydroxyl chain being close to the water interface and the side of polyene portion facing to the micelle interior. CD measurements demonstrate that AmB is in a monomeric form in SDS micelles. The structure of AmB in the micelles obtained in the present study may reproduce the initial stage of membrane interaction of AmB prior to the assembly formation in biomembranes.

Original languageEnglish
Pages (from-to)700-706
Number of pages7
JournalJournal of Organic Chemistry
Volume72
Issue number3
DOIs
Publication statusPublished - Feb 2 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

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