Conformational change of protein due to contact with bioceramic materials

S. Nagai; K. Tsuru; S. Hayakawa; A. Osaka

doi:10.4028/0-87849-961-x.517

Conformational change of protein due to contact with bioceramic materials

S. Nagai, K. Tsuru, S. Hayakawa, A. Osaka

Research output: Contribution to journal › Article › peer-review

Abstract

The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al₂O₃, SiO₂, TiO₂, ZrO₂, hydroxyapatite and β-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO₂, ZrO₂ and Al₂O₃, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO₂ and ZrO₂. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.

Original language	English
Pages (from-to)	517-520
Number of pages	4
Journal	Key Engineering Materials
Volume	284-286
DOIs	https://doi.org/10.4028/0-87849-961-x.517
Publication status	Published - 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

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title = "Conformational change of protein due to contact with bioceramic materials",

abstract = "The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and β-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.",

author = "S. Nagai and K. Tsuru and S. Hayakawa and A. Osaka",

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T1 - Conformational change of protein due to contact with bioceramic materials

AU - Nagai, S.

AU - Tsuru, K.

AU - Hayakawa, S.

AU - Osaka, A.

PY - 2005

Y1 - 2005

N2 - The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and β-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.

AB - The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and β-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.

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Conformational change of protein due to contact with bioceramic materials

Abstract

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