Conformational change of protein due to contact with bioceramic materials

S. Nagai, K. Tsuru, S. Hayakawa, A. Osaka

Research output: Contribution to journalArticle

Abstract

The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and β-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.

Original languageEnglish
Pages (from-to)517-520
Number of pages4
JournalKey Engineering Materials
Volume284-286
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Circular dichroism spectroscopy
Bioceramics
Bovine Serum Albumin
Proteins
Powders
Hydroxyapatite
Contacts (fluid mechanics)
Phosphates
Agglomeration
Adsorption
Durapatite

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Nagai, S., Tsuru, K., Hayakawa, S., & Osaka, A. (2005). Conformational change of protein due to contact with bioceramic materials. Key Engineering Materials, 284-286, 517-520.

Conformational change of protein due to contact with bioceramic materials. / Nagai, S.; Tsuru, K.; Hayakawa, S.; Osaka, A.

In: Key Engineering Materials, Vol. 284-286, 2005, p. 517-520.

Research output: Contribution to journalArticle

Nagai, S, Tsuru, K, Hayakawa, S & Osaka, A 2005, 'Conformational change of protein due to contact with bioceramic materials', Key Engineering Materials, vol. 284-286, pp. 517-520.
Nagai, S. ; Tsuru, K. ; Hayakawa, S. ; Osaka, A. / Conformational change of protein due to contact with bioceramic materials. In: Key Engineering Materials. 2005 ; Vol. 284-286. pp. 517-520.
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