Conformational transition of giant DNA in a confined space surrounded by a phospholipid membrane

Ayako Kato, Eri Shindo, Takahiro Sakaue, Akihiko Tsuji, Kenichi Yoshikawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

It has been established that a long DNA molecule exhibits a large discrete conformational change from a coiled state to a highly folded state in aqueous solution, depending on the presence of various condensing agents such as polyamines. In this study, T4 DNA labeled with fluorescent dyes was encapsulated in a cell-sized microdroplet covered with a phospholipid membrane to investigate the conformational behavior of a DNA molecule in such a confined space. Fluorescence microscopy showed that the presence of Mg2+ induced the adsorption of DNA onto the membrane inner-surface of a droplet composed of phosphatidylethanolamine, while no adsorption was observed onto a phosphatidylcholine membrane. Under the presence of spermine (tetravalent amine), DNAhad a folded conformation in the bulk solution. However, when these molecules were encapsulated in the microdroplet, DNA adsorbed onto the membrane surface accompanied by unfolding of its structure into an extended coil conformation under high concentrations of Mg2+. In addition, DNA molecules trapped in large droplets tended not to be adsorbed on the membrane, i.e., no conformational transition occurred. A thermodynamic analysis suggests that the translational entropy loss of a DNA molecule that is accompanied by adsorption is a key factor in these phenomena under micrometer-scale confinement.

Original languageEnglish
Pages (from-to)1678-1686
Number of pages9
JournalBiophysical Journal
Volume97
Issue number6
DOIs
Publication statusPublished - Sep 16 2009

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Confined Spaces
Phospholipids
Membranes
DNA
Adsorption
Spermine
Polyamines
Entropy
Phosphatidylcholines
Fluorescent Dyes
Thermodynamics
Fluorescence Microscopy
Amines

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Conformational transition of giant DNA in a confined space surrounded by a phospholipid membrane. / Kato, Ayako; Shindo, Eri; Sakaue, Takahiro; Tsuji, Akihiko; Yoshikawa, Kenichi.

In: Biophysical Journal, Vol. 97, No. 6, 16.09.2009, p. 1678-1686.

Research output: Contribution to journalArticle

Kato, Ayako ; Shindo, Eri ; Sakaue, Takahiro ; Tsuji, Akihiko ; Yoshikawa, Kenichi. / Conformational transition of giant DNA in a confined space surrounded by a phospholipid membrane. In: Biophysical Journal. 2009 ; Vol. 97, No. 6. pp. 1678-1686.
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