Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

Hideaki Toda, Yasutaka Saito, Takuhito Koike, Fumio Takizawa, Kyosuke Araki, Takeshi Yabu, Tomonori Somamoto, Hiroaki Suetake, Yuzuru Suzuki, Mitsuru Ototake, Tadaaki Moritomo, Teruyuki Nakanishi

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The presence of helper and cytotoxic T cells in fish has been suggested, although T cell subsets have yet to be identified at the cellular level. In order to investigate the functions of CD4 and CD8α positive T cells we attempted to produce and characterize monoclonal antibodies (mAbs) against teleost CD4 and CD8α. Here we report the successful production of mAbs against CD4 and CD8α in clonal ginbuna crucian carp Carassius auratus langsdorfii and the function of CD4 positive T cells. In this study we demonstrate the presence of teleost CD4- and CD8α-positive T cell subsets with morphology, tissue distribution and gene expression similar to those of mammalian CD4- and CD8-positive T lymphocytes. Using mAbs we found that CD4/CD8 double positive T cells are only present in the thymus, suggesting that it is the site of T cell development. We further demonstrated in vitro proliferation of CD4 positive T cells by allogeneic combination of mixed leukocyte culture and antigen-specific proliferation of CD4 positive T cells after in vitro sensitization with OVA. In our previous study we showed that CD8α-positive lymphocytes are the primary cell type showing specific cytotoxicity against allogeneic targets. Collectively, these findings suggest that CD4 and CD8α positive T cells in ginbuna are equivalent to helper and cytotoxic T lymphocytes (CTL) in mammals, respectively. This is the first report to show the characteristics and functions of CD4 positive T cells in fish and these findings shed light into the evolutionary origins and primordial functions of helper T cells.

Original languageEnglish
Pages (from-to)650-660
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume35
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

CD4-Positive T-Lymphocytes
Fishes
T-Lymphocytes
Helper-Inducer T-Lymphocytes
CD8-Positive T-Lymphocytes
Monoclonal Antibodies
T-Lymphocyte Subsets
Goldfish
Carps
Cytotoxic T-Lymphocytes
Tissue Distribution
HLA Antigens
Thymus Gland
Mammals
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish. / Toda, Hideaki; Saito, Yasutaka; Koike, Takuhito; Takizawa, Fumio; Araki, Kyosuke; Yabu, Takeshi; Somamoto, Tomonori; Suetake, Hiroaki; Suzuki, Yuzuru; Ototake, Mitsuru; Moritomo, Tadaaki; Nakanishi, Teruyuki.

In: Developmental and Comparative Immunology, Vol. 35, No. 6, 01.06.2011, p. 650-660.

Research output: Contribution to journalArticle

Toda, H, Saito, Y, Koike, T, Takizawa, F, Araki, K, Yabu, T, Somamoto, T, Suetake, H, Suzuki, Y, Ototake, M, Moritomo, T & Nakanishi, T 2011, 'Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish', Developmental and Comparative Immunology, vol. 35, no. 6, pp. 650-660. https://doi.org/10.1016/j.dci.2011.01.013
Toda, Hideaki ; Saito, Yasutaka ; Koike, Takuhito ; Takizawa, Fumio ; Araki, Kyosuke ; Yabu, Takeshi ; Somamoto, Tomonori ; Suetake, Hiroaki ; Suzuki, Yuzuru ; Ototake, Mitsuru ; Moritomo, Tadaaki ; Nakanishi, Teruyuki. / Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish. In: Developmental and Comparative Immunology. 2011 ; Vol. 35, No. 6. pp. 650-660.
@article{152e4d54e602407e8a5e94978e820375,
title = "Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish",
abstract = "The presence of helper and cytotoxic T cells in fish has been suggested, although T cell subsets have yet to be identified at the cellular level. In order to investigate the functions of CD4 and CD8α positive T cells we attempted to produce and characterize monoclonal antibodies (mAbs) against teleost CD4 and CD8α. Here we report the successful production of mAbs against CD4 and CD8α in clonal ginbuna crucian carp Carassius auratus langsdorfii and the function of CD4 positive T cells. In this study we demonstrate the presence of teleost CD4- and CD8α-positive T cell subsets with morphology, tissue distribution and gene expression similar to those of mammalian CD4- and CD8-positive T lymphocytes. Using mAbs we found that CD4/CD8 double positive T cells are only present in the thymus, suggesting that it is the site of T cell development. We further demonstrated in vitro proliferation of CD4 positive T cells by allogeneic combination of mixed leukocyte culture and antigen-specific proliferation of CD4 positive T cells after in vitro sensitization with OVA. In our previous study we showed that CD8α-positive lymphocytes are the primary cell type showing specific cytotoxicity against allogeneic targets. Collectively, these findings suggest that CD4 and CD8α positive T cells in ginbuna are equivalent to helper and cytotoxic T lymphocytes (CTL) in mammals, respectively. This is the first report to show the characteristics and functions of CD4 positive T cells in fish and these findings shed light into the evolutionary origins and primordial functions of helper T cells.",
author = "Hideaki Toda and Yasutaka Saito and Takuhito Koike and Fumio Takizawa and Kyosuke Araki and Takeshi Yabu and Tomonori Somamoto and Hiroaki Suetake and Yuzuru Suzuki and Mitsuru Ototake and Tadaaki Moritomo and Teruyuki Nakanishi",
year = "2011",
month = "6",
day = "1",
doi = "10.1016/j.dci.2011.01.013",
language = "English",
volume = "35",
pages = "650--660",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

AU - Toda, Hideaki

AU - Saito, Yasutaka

AU - Koike, Takuhito

AU - Takizawa, Fumio

AU - Araki, Kyosuke

AU - Yabu, Takeshi

AU - Somamoto, Tomonori

AU - Suetake, Hiroaki

AU - Suzuki, Yuzuru

AU - Ototake, Mitsuru

AU - Moritomo, Tadaaki

AU - Nakanishi, Teruyuki

PY - 2011/6/1

Y1 - 2011/6/1

N2 - The presence of helper and cytotoxic T cells in fish has been suggested, although T cell subsets have yet to be identified at the cellular level. In order to investigate the functions of CD4 and CD8α positive T cells we attempted to produce and characterize monoclonal antibodies (mAbs) against teleost CD4 and CD8α. Here we report the successful production of mAbs against CD4 and CD8α in clonal ginbuna crucian carp Carassius auratus langsdorfii and the function of CD4 positive T cells. In this study we demonstrate the presence of teleost CD4- and CD8α-positive T cell subsets with morphology, tissue distribution and gene expression similar to those of mammalian CD4- and CD8-positive T lymphocytes. Using mAbs we found that CD4/CD8 double positive T cells are only present in the thymus, suggesting that it is the site of T cell development. We further demonstrated in vitro proliferation of CD4 positive T cells by allogeneic combination of mixed leukocyte culture and antigen-specific proliferation of CD4 positive T cells after in vitro sensitization with OVA. In our previous study we showed that CD8α-positive lymphocytes are the primary cell type showing specific cytotoxicity against allogeneic targets. Collectively, these findings suggest that CD4 and CD8α positive T cells in ginbuna are equivalent to helper and cytotoxic T lymphocytes (CTL) in mammals, respectively. This is the first report to show the characteristics and functions of CD4 positive T cells in fish and these findings shed light into the evolutionary origins and primordial functions of helper T cells.

AB - The presence of helper and cytotoxic T cells in fish has been suggested, although T cell subsets have yet to be identified at the cellular level. In order to investigate the functions of CD4 and CD8α positive T cells we attempted to produce and characterize monoclonal antibodies (mAbs) against teleost CD4 and CD8α. Here we report the successful production of mAbs against CD4 and CD8α in clonal ginbuna crucian carp Carassius auratus langsdorfii and the function of CD4 positive T cells. In this study we demonstrate the presence of teleost CD4- and CD8α-positive T cell subsets with morphology, tissue distribution and gene expression similar to those of mammalian CD4- and CD8-positive T lymphocytes. Using mAbs we found that CD4/CD8 double positive T cells are only present in the thymus, suggesting that it is the site of T cell development. We further demonstrated in vitro proliferation of CD4 positive T cells by allogeneic combination of mixed leukocyte culture and antigen-specific proliferation of CD4 positive T cells after in vitro sensitization with OVA. In our previous study we showed that CD8α-positive lymphocytes are the primary cell type showing specific cytotoxicity against allogeneic targets. Collectively, these findings suggest that CD4 and CD8α positive T cells in ginbuna are equivalent to helper and cytotoxic T lymphocytes (CTL) in mammals, respectively. This is the first report to show the characteristics and functions of CD4 positive T cells in fish and these findings shed light into the evolutionary origins and primordial functions of helper T cells.

UR - http://www.scopus.com/inward/record.url?scp=79952449676&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952449676&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2011.01.013

DO - 10.1016/j.dci.2011.01.013

M3 - Article

C2 - 21272597

AN - SCOPUS:79952449676

VL - 35

SP - 650

EP - 660

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 6

ER -