Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts

Hiroki Akizawa, Hiroaki Nagatomo, Haruka Odagiri, Nanami Kohri, Nobuhiko Yamauchi, Yojiro Yanagawa, Masashi Nagano, Masashi Takahashi, Manabu Kawahara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A common process during preimplantation mammalian development is blastocyst formation, which utilizes signaling through fibroblast growth factor receptor 2 (FGFR2), yet the mechanisms through which FGFR2 signaling affect preimplantation development in bovine embryos remain incompletely understood. Here, we used RNA-interference to investigate the in vitro development, the frequency of blastomere apoptosis, and the mRNA expression of developmental marker genes in FGF receptor 2-knockdown (FGFR2-KD) bovine embryos. A reduction in FGFR2 mRNA did not affect preimplantation development or the frequency of apoptotic blastomeres, but did enhanced proliferation of the inner cell mass in blastocysts (P < 0.05)—which differs from the phenotype reported for bovine embryos using a pharmacological approach (treatment with the pan-FGFR blocker PD173074), but agrees with previous results obtained using mouse embryos. Moreover, the expression of an epiblast marker gene, NANOG, and a primitive endoderm marker gene, GATA6, remained unchanged, whereas the expression of another primitive endoderm marker gene, HNF4A, was significantly reduced in FGFR2-KD embryos. Therefore, FGFR2 signaling appears to be associated with the regulation of inner cell mass development and proliferation during blastocyst formation in cattle. Mol. Reprod. Dev. 83: 516–525, 2016.

Original languageEnglish
Pages (from-to)516-525
Number of pages10
JournalMolecular Reproduction and Development
Volume83
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Receptor, Fibroblast Growth Factor, Type 2
Blastocyst
Embryonic Structures
Blastomeres
Endoderm
Blastocyst Inner Cell Mass
Genes
Developmental Genes
Fibroblast Growth Factor Receptors
Germ Layers
Messenger RNA
Fibroblast Growth Factor 2
RNA Interference
Pharmacology
Apoptosis
Phenotype

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts. / Akizawa, Hiroki; Nagatomo, Hiroaki; Odagiri, Haruka; Kohri, Nanami; Yamauchi, Nobuhiko; Yanagawa, Yojiro; Nagano, Masashi; Takahashi, Masashi; Kawahara, Manabu.

In: Molecular Reproduction and Development, Vol. 83, No. 6, 01.06.2016, p. 516-525.

Research output: Contribution to journalArticle

Akizawa, Hiroki ; Nagatomo, Hiroaki ; Odagiri, Haruka ; Kohri, Nanami ; Yamauchi, Nobuhiko ; Yanagawa, Yojiro ; Nagano, Masashi ; Takahashi, Masashi ; Kawahara, Manabu. / Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts. In: Molecular Reproduction and Development. 2016 ; Vol. 83, No. 6. pp. 516-525.
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