Conserved sequence motifs in the small subunit of human general transcription factor TFIIE

Hideki Sumimoto, Yoshiaki Ohkuma, Eric Sinn, Hiroyuki Kato, Shunichi Shimasaki, Masami Horikoshi, Robert G. Roeder

Research output: Contribution to journalArticle

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Abstract

A GENERAL initiation factor, TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors1,2. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-α) and two of 34,000 (TFIIE-β)3,4. TFIIE-β is required in conjunction with TFIIE-α for transcription initiation. Here we report the cloning and expression of a complementary DNA encoding a functional human TFIIE-β. Recombinant TFIIE-β could replace the natural TFIIE-β for transcription in conjunction with TFIIE-α. Amino-acid sequence comparisons reveal regions with sequence similarities to: subregion 3 of bacterial factors6; a region of RAP30 (the small subunit of TFIIF) with sequence similarity to a a factor subregion implicated in binding to RNA polymerase7; and a portion of the basic region-helix-loop-helix motif found in several enhancer-binding proteins8,10. These potential homologies have implications for the role of TFIIE in preinitiation complex assembly and function.

Original languageEnglish
Pages (from-to)401-404
Number of pages4
JournalNature
Volume354
Issue number6352
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

Fingerprint

General Transcription Factors
Conserved Sequence
Helix-Loop-Helix Motifs
transcription factor TFIIE
Peptide Initiation Factors
RNA Polymerase II
Organism Cloning
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • General

Cite this

Sumimoto, H., Ohkuma, Y., Sinn, E., Kato, H., Shimasaki, S., Horikoshi, M., & Roeder, R. G. (1991). Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. Nature, 354(6352), 401-404. https://doi.org/10.1038/354401a0

Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. / Sumimoto, Hideki; Ohkuma, Yoshiaki; Sinn, Eric; Kato, Hiroyuki; Shimasaki, Shunichi; Horikoshi, Masami; Roeder, Robert G.

In: Nature, Vol. 354, No. 6352, 01.01.1991, p. 401-404.

Research output: Contribution to journalArticle

Sumimoto, H, Ohkuma, Y, Sinn, E, Kato, H, Shimasaki, S, Horikoshi, M & Roeder, RG 1991, 'Conserved sequence motifs in the small subunit of human general transcription factor TFIIE', Nature, vol. 354, no. 6352, pp. 401-404. https://doi.org/10.1038/354401a0
Sumimoto H, Ohkuma Y, Sinn E, Kato H, Shimasaki S, Horikoshi M et al. Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. Nature. 1991 Jan 1;354(6352):401-404. https://doi.org/10.1038/354401a0
Sumimoto, Hideki ; Ohkuma, Yoshiaki ; Sinn, Eric ; Kato, Hiroyuki ; Shimasaki, Shunichi ; Horikoshi, Masami ; Roeder, Robert G. / Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. In: Nature. 1991 ; Vol. 354, No. 6352. pp. 401-404.
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