Conserved sequence motifs in the small subunit of human general transcription factor TFIIE

Hideki Sumimoto, Yoshiaki Ohkuma, Eric Sinn, Hiroyuki Kato, Shunichi Shimasaki, Masami Horikoshi, Robert G. Roeder

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

A GENERAL initiation factor, TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors1,2. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-α) and two of 34,000 (TFIIE-β)3,4. TFIIE-β is required in conjunction with TFIIE-α for transcription initiation. Here we report the cloning and expression of a complementary DNA encoding a functional human TFIIE-β. Recombinant TFIIE-β could replace the natural TFIIE-β for transcription in conjunction with TFIIE-α. Amino-acid sequence comparisons reveal regions with sequence similarities to: subregion 3 of bacterial factors6; a region of RAP30 (the small subunit of TFIIF) with sequence similarity to a a factor subregion implicated in binding to RNA polymerase7; and a portion of the basic region-helix-loop-helix motif found in several enhancer-binding proteins8,10. These potential homologies have implications for the role of TFIIE in preinitiation complex assembly and function.

Original languageEnglish
Pages (from-to)401-404
Number of pages4
JournalNature
Volume354
Issue number6352
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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