Constitutive expression of phosphoketolase, a key enzyme for metabolic shift from homo- to heterolactic fermentation in Enterococcus mundtii QU 25

Keisuke Nabeta, Satoru Watanabe, Taku Chibazakura, Takeshi Zendo, Kenji Sonomoto, Mariko Shimizu-Kadota, Hirofumi Yoshikawa

Research output: Contribution to journalComment/debatepeer-review

Abstract

Phosphoketolase (PK) is responsible for heterolactic fermentation; however, the PK gene of Enterococcus mundtii QU 25, xfpA, is transcribed constitutively, even under homolactic fermentation conditions. In order to deduce the regulatory mechanisms of PK activity in QU 25, XfpA levels in QU 25 cells under hetero- and homolactic fermentation conditions were tested using western blotting. The results showed that the XfpA protein expression was similar under both conditions and that the expression products formed complexes, most likely homodimers, indicating that the regulation of PK activity is downstream of translation.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalBioscience of Microbiota, Food and Health
Volume38
Issue number3
DOIs
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Gastroenterology

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