Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir; Hirofumi Ichinose; Hiroyuki Wariishi

doi:10.1128/AEM.02491-10

Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir, Hirofumi Ichinose, Hiroyuki Wariishi

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22 Citations (Scopus)

Abstract

A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

Original language	English
Pages (from-to)	3147-3150
Number of pages	4
Journal	Applied and environmental microbiology
Volume	77
Issue number	9
DOIs	https://doi.org/10.1128/AEM.02491-10
Publication status	Published - May 1 2011

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All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Cite this

Nazmul Hussain Nazir, K. H. M., Ichinose, H., & Wariishi, H. (2011). Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae. Applied and environmental microbiology, 77(9), 3147-3150. https://doi.org/10.1128/AEM.02491-10

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10.1128/AEM.02491-10