Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir, Hirofumi Ichinose, Hiroyuki Wariishi

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22 Citations (Scopus)

Abstract

A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

Original languageEnglish
Pages (from-to)3147-3150
Number of pages4
JournalApplied and environmental microbiology
Volume77
Issue number9
DOIs
Publication statusPublished - May 1 2011

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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