TY - JOUR
T1 - Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae
AU - Nazmul Hussain Nazir, K. H.M.
AU - Ichinose, Hirofumi
AU - Wariishi, Hiroyuki
PY - 2011/5
Y1 - 2011/5
N2 - A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.
AB - A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.
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U2 - 10.1128/AEM.02491-10
DO - 10.1128/AEM.02491-10
M3 - Article
C2 - 21378053
AN - SCOPUS:79955593960
VL - 77
SP - 3147
EP - 3150
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 9
ER -