Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite

Michiko Terada, Kenji Izumi, Hisashi Ohnuki, Taro Saito, Hiroko Kato, Marie Yamamoto, Yoshiro Kawano, Kayoko Nozawa-Inoue, Haruhiko Kashiwazaki, Toshiyuki Ikoma, Junzo Tanaka, Takeyasu Maeda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study was designed to (1) assess the in vitro biocompatibility of a chitosan-collagen composite scaffold (C3) constructed by blending commercial chitosan and tilapia scale collagen with oral mucosa keratinocytes, (2) histologically and immunohistochemically characterize an ex vivo-produced oral mucosa equivalent constructed using the C3 (EVPOME-C), and (3) compare EVPOME-C with oral mucosa constructs utilizing AlloDerm® (EVPOME-A), BioMend® Extend™ (EVPOME-B), and native oral mucosa. C3 scaffold had a well-developed fibril network and a sufficiently small porosity to prevent keratinocytes from growing inside the scaffold after cell-seeding. The EVPOME oral mucosa constructs were fabricated in a chemically defined culture system. After culture at an air-liquid interface, EVPOME-C and EVPOME-B had multilayered epithelium with keratinization, while EVPOME-A had a more organized stratified epithelium. Ki-67 and p63 immunolabeled cells in the basal layer of all EVPOMEs suggested a regenerative ability. Compared with native oral mucosa, the keratin 15 and 10/13 expression patterns in all EVPOMEs showed a less-organized differentiation pattern. In contrast to the β1-integrin and laminin distribution in EVPOME-A and native oral mucosa, the subcellular deposition in EVPOME-C and EVPOME-B indicated that complete basement membrane formation failed. These findings demonstrated that C3 has a potential application for epithelial tissue engineering and provides a new potential therapeutic device for oral mucosa regenerative medicine. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.

Original languageEnglish
Pages (from-to)1792-1802
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume100 B
Issue number7
DOIs
Publication statusPublished - Oct 1 2012
Externally publishedYes

Fingerprint

Chitosan
Collagen
Fish
Tissue
Composite materials
Bioelectric potentials
Scaffolds
Keratin-15
Mucous Membrane
Keratin-10
Keratin
Laminin
Scaffolds (biology)
Biocompatibility
Tissue engineering
Integrins
Porosity

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite. / Terada, Michiko; Izumi, Kenji; Ohnuki, Hisashi; Saito, Taro; Kato, Hiroko; Yamamoto, Marie; Kawano, Yoshiro; Nozawa-Inoue, Kayoko; Kashiwazaki, Haruhiko; Ikoma, Toshiyuki; Tanaka, Junzo; Maeda, Takeyasu.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 100 B, No. 7, 01.10.2012, p. 1792-1802.

Research output: Contribution to journalArticle

Terada, M, Izumi, K, Ohnuki, H, Saito, T, Kato, H, Yamamoto, M, Kawano, Y, Nozawa-Inoue, K, Kashiwazaki, H, Ikoma, T, Tanaka, J & Maeda, T 2012, 'Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite', Journal of Biomedical Materials Research - Part B Applied Biomaterials, vol. 100 B, no. 7, pp. 1792-1802. https://doi.org/10.1002/jbm.b.32746
Terada, Michiko ; Izumi, Kenji ; Ohnuki, Hisashi ; Saito, Taro ; Kato, Hiroko ; Yamamoto, Marie ; Kawano, Yoshiro ; Nozawa-Inoue, Kayoko ; Kashiwazaki, Haruhiko ; Ikoma, Toshiyuki ; Tanaka, Junzo ; Maeda, Takeyasu. / Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2012 ; Vol. 100 B, No. 7. pp. 1792-1802.
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