Construction and Gilding of Metal-Organic Frameworks and Microtubule Conjugates

Metal-organic frameworks (MOFs) are well-ordered microporous crystalline materials synthesized from metal cluster nodes and organic ligands. Using organic ligands with functional and reactive moieties enables post-synthetic modification (PSM) for further functionalization of MOFs. In this study, bioconjugation of MOFs with functional motor proteins, such as kinesin-microtubule (MT) system was investigated by employing PSM. Kinesin-MT system, energy diffusive gliding motion of MT was performed by consuming chemical energy of adenosine-tri-phosphate (ATP) in vitro. Thus, we attempted establishment of gliding of MOF by applying bio-motor system, in which reconstituted MT filaments are propelled over a kinesin-coated substrate in the presence of ATP.