Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter

Miyako Udono, Tsukasa Fujiki, Makiko Yamashita, Kazuyuki Katsuki, Masaaki Funata, Yusuke Imada, Kaichi Yoshizaki, Sanetaka Shirahata, Yoshinori Katakura

Research output: Contribution to journalArticle

Abstract

We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.

Original languageEnglish
Pages (from-to)1638-1641
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number6
DOIs
Publication statusPublished - Jul 1 2008

Fingerprint

telomerase
RNA-directed DNA polymerase
Genes
Neoplasm Genes
promoter regions
Tetracycline
tetracycline
Gene expression
neoplasms
Gene Expression
Gene therapy
Neoplasms
gene expression
doxycycline
Trans-Activators
gene therapy
genes
Doxycycline
response elements
Response Elements

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter. / Udono, Miyako; Fujiki, Tsukasa; Yamashita, Makiko; Katsuki, Kazuyuki; Funata, Masaaki; Imada, Yusuke; Yoshizaki, Kaichi; Shirahata, Sanetaka; Katakura, Yoshinori.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 6, 01.07.2008, p. 1638-1641.

Research output: Contribution to journalArticle

Udono, Miyako ; Fujiki, Tsukasa ; Yamashita, Makiko ; Katsuki, Kazuyuki ; Funata, Masaaki ; Imada, Yusuke ; Yoshizaki, Kaichi ; Shirahata, Sanetaka ; Katakura, Yoshinori. / Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 6. pp. 1638-1641.
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