TY - JOUR
T1 - Construction of a series of pACYC-derived plasmid vectors
AU - Nakano, Yoshio
AU - Yoshida, Yasuo
AU - Yamashita, Yoshihisa
AU - Koga, Toshihiko
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research (05454563) from the Ministry of Education, Science, and Culture of Japan and by a research grant from the Japan Foundation for Aging and Health.
PY - 1995/8/30
Y1 - 1995/8/30
N2 - A series of cloning vectors has been constructed from pACYC177 and pACYC184. These vectors contain a MCS, the lacZα reporter gene, and P15A ori. Plasmids pMBL18, pMBL19 and pMBLcos express the bla gene encoding ampicillin resistance, while pMCL200 and pMCL210 contain the cat gene encoding chloramphenicol resistance. In addition, the cosmid pMBLcos was constructed with the P15A ori. These vectors are useful for cloning or subcloning genes which are unstable and/or toxic in high-copy-number vectors.
AB - A series of cloning vectors has been constructed from pACYC177 and pACYC184. These vectors contain a MCS, the lacZα reporter gene, and P15A ori. Plasmids pMBL18, pMBL19 and pMBLcos express the bla gene encoding ampicillin resistance, while pMCL200 and pMCL210 contain the cat gene encoding chloramphenicol resistance. In addition, the cosmid pMBLcos was constructed with the P15A ori. These vectors are useful for cloning or subcloning genes which are unstable and/or toxic in high-copy-number vectors.
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U2 - 10.1016/0378-1119(95)00320-6
DO - 10.1016/0378-1119(95)00320-6
M3 - Article
C2 - 7557406
AN - SCOPUS:0029085552
SN - 0378-1119
VL - 162
SP - 157
EP - 158
JO - Gene
JF - Gene
IS - 1
ER -