Construction of an energy transfer system in the bio-nanocup space by heteromeric assembly of gp27 and gp5 proteins isolated from bacteriophage T4

Tomomi Koshiyama, Takafumi Ueno, Shuji Kanamaru, Fumio Arisaka, Yoshihito Watanabe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Protein assemblies, such as viruses and ferritins, have been employed as useful molecular templates for the accumulation of organic and inorganic compounds to construct bio-nanomaterials. While several methods for conjugation of heterofunctional molecules with protein assemblies have been reported, it remains difficult to control their fixation sites in the assemblies. In this article, we demonstrate the three-dimensional arrangement of different types of fluorescent probes using the heteromeric self-assembly of (gp27-gp5)3 which is the component protein of bacteriophage T4 (gp: gene product). The composites exhibited fluorescence resonance energy transfer from fluorescein to tetramethylrhodamine dyes immobilized in the bio-nanocup space. The alternation of the donor and acceptor positions induced fluorescence self-quenching by the formation of ground-state complexes of the acceptors. These results indicate that the site-specific conjugation method using the bio-nanocup space of the heteromeric protein assembly has potential for the integration of several types of functional molecules in protein nanospaces.

Original languageEnglish
Pages (from-to)2649-2654
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume7
Issue number12
DOIs
Publication statusPublished - Jul 20 2009

Fingerprint

bacteriophages
Bacteriophage T4
Bacteriophages
Energy Transfer
Energy transfer
assembly
energy transfer
proteins
assemblies
Proteins
conjugation
Inorganic compounds
inorganic compounds
Fluorescence Resonance Energy Transfer
Molecules
Nanostructures
alternations
viruses
resonance fluorescence
Ferritins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Construction of an energy transfer system in the bio-nanocup space by heteromeric assembly of gp27 and gp5 proteins isolated from bacteriophage T4. / Koshiyama, Tomomi; Ueno, Takafumi; Kanamaru, Shuji; Arisaka, Fumio; Watanabe, Yoshihito.

In: Organic and Biomolecular Chemistry, Vol. 7, No. 12, 20.07.2009, p. 2649-2654.

Research output: Contribution to journalArticle

Koshiyama, Tomomi ; Ueno, Takafumi ; Kanamaru, Shuji ; Arisaka, Fumio ; Watanabe, Yoshihito. / Construction of an energy transfer system in the bio-nanocup space by heteromeric assembly of gp27 and gp5 proteins isolated from bacteriophage T4. In: Organic and Biomolecular Chemistry. 2009 ; Vol. 7, No. 12. pp. 2649-2654.
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