Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites

Hee Jin Jeong, Kenji Matsumoto, Shuya Itayama, Kozue Kodama, Ryoji Abe, Jinhua Dong, Mitsuru Shindo, Hiroshi Ueda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We successfully converted an antibody single-chain variable fragment and a full-sized antibody to Quenchbodies, which are a type of powerful fluorescent immunosensor, through ultraviolet-based photochemical crosslinking of an indole-3-butyric acid-conjugated fluorescent dye to the nucleotide-binding sites near the antigen-binding sites.

Original languageEnglish
Pages (from-to)10200-10203
Number of pages4
JournalChemical Communications
Volume53
Issue number73
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Binding sites
Nucleotides
Antibodies
Crosslinking
Coloring Agents
Dyes
Binding Sites
Immunosensors
Single-Chain Antibodies
Butyric acid
Antigens
Fluorescent Dyes
indolebutyric acid

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Jeong, H. J., Matsumoto, K., Itayama, S., Kodama, K., Abe, R., Dong, J., ... Ueda, H. (2017). Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites. Chemical Communications, 53(73), 10200-10203. https://doi.org/10.1039/c7cc03043f

Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites. / Jeong, Hee Jin; Matsumoto, Kenji; Itayama, Shuya; Kodama, Kozue; Abe, Ryoji; Dong, Jinhua; Shindo, Mitsuru; Ueda, Hiroshi.

In: Chemical Communications, Vol. 53, No. 73, 01.01.2017, p. 10200-10203.

Research output: Contribution to journalArticle

Jeong, HJ, Matsumoto, K, Itayama, S, Kodama, K, Abe, R, Dong, J, Shindo, M & Ueda, H 2017, 'Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites', Chemical Communications, vol. 53, no. 73, pp. 10200-10203. https://doi.org/10.1039/c7cc03043f
Jeong, Hee Jin ; Matsumoto, Kenji ; Itayama, Shuya ; Kodama, Kozue ; Abe, Ryoji ; Dong, Jinhua ; Shindo, Mitsuru ; Ueda, Hiroshi. / Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites. In: Chemical Communications. 2017 ; Vol. 53, No. 73. pp. 10200-10203.
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