Construction of multi-layered cardiomyocyte sheets using magnetite nanoparticles and magnetic force

Kazunori Shimizu, Akira Ito, Jong Kook Lee, Tatsuro Yoshida, Keiko Miwa, Hisaaki Ishiguro, Yasushi Numaguchi, Toyoaki Murohara, Itsuo Kodama, Hiroyuki Honda

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Heart tissue engineering requires construction of three-dimensional (3-D) tissues composed of cardiomyocytes (CMs) that are tightly connected to each other. The aim of this study was to construct "scaffold-less" multilayered 3-D CM sheets using magnetic force-based tissue engineering (Mag-TE) and to evaluate the cell-to-cell functional connections within the CM sheets. Original magnetite cationic liposomes (MCLs) with a positive surface charge (which facilitate adsorption to the target cell surface) were taken up by CMs that were isolated from 2-day-old Wistar rats. When MCLs were added to the medium of CMs at magnetite concentrations of 25, 50, and 100 pg per cell, subsequent measurements showed that 7.2, 13.2, and 27.3 pg of magnetite were taken up per cell, respectively, after 4 h incubation at 37°C. Further, no toxicity was observed after a 24 h incubation period. Using magnetically labeled CMs (magnetite concentration, 100 pg/cell), multi-layered CM sheets were constructed. Immunofluorescent staining of connexin43 demonstrated the presence of gap junctions within the CM sheets that were constructed by Mag-TE. Moreover, electrical connections within the CM sheets constructed by Mag-TE were confirmed using extracellular potential mapping. These results indicate that Mag-TE is a viable methodology for heart tissue engineering.

Original languageEnglish
Pages (from-to)803-809
Number of pages7
JournalBiotechnology and Bioengineering
Volume96
Issue number4
DOIs
Publication statusPublished - Mar 1 2007
Externally publishedYes

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
Ferrosoferric Oxide
Tissue engineering
Cardiac Myocytes
Magnetite
Tissue Engineering
Liposomes
Connexin 43
Bioelectric potentials
Surface charge
Scaffolds
Toxicity
Rats
Gap Junctions
Tissue
Adsorption
Wistar Rats

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Shimizu, K., Ito, A., Lee, J. K., Yoshida, T., Miwa, K., Ishiguro, H., ... Honda, H. (2007). Construction of multi-layered cardiomyocyte sheets using magnetite nanoparticles and magnetic force. Biotechnology and Bioengineering, 96(4), 803-809. https://doi.org/10.1002/bit.21094

Construction of multi-layered cardiomyocyte sheets using magnetite nanoparticles and magnetic force. / Shimizu, Kazunori; Ito, Akira; Lee, Jong Kook; Yoshida, Tatsuro; Miwa, Keiko; Ishiguro, Hisaaki; Numaguchi, Yasushi; Murohara, Toyoaki; Kodama, Itsuo; Honda, Hiroyuki.

In: Biotechnology and Bioengineering, Vol. 96, No. 4, 01.03.2007, p. 803-809.

Research output: Contribution to journalArticle

Shimizu, K, Ito, A, Lee, JK, Yoshida, T, Miwa, K, Ishiguro, H, Numaguchi, Y, Murohara, T, Kodama, I & Honda, H 2007, 'Construction of multi-layered cardiomyocyte sheets using magnetite nanoparticles and magnetic force', Biotechnology and Bioengineering, vol. 96, no. 4, pp. 803-809. https://doi.org/10.1002/bit.21094
Shimizu, Kazunori ; Ito, Akira ; Lee, Jong Kook ; Yoshida, Tatsuro ; Miwa, Keiko ; Ishiguro, Hisaaki ; Numaguchi, Yasushi ; Murohara, Toyoaki ; Kodama, Itsuo ; Honda, Hiroyuki. / Construction of multi-layered cardiomyocyte sheets using magnetite nanoparticles and magnetic force. In: Biotechnology and Bioengineering. 2007 ; Vol. 96, No. 4. pp. 803-809.
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