Construction of piggyBac-based vectors using visible and drug-resistance marker for introducing foreign genes into silkworm cultured cells

Tsuneyuki Tatsuke, Sun Mee Hong, Hiromi Tobata, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transgenic organisms have been indispensable for modern genetic analysis, such as over expression or knocked-down of the genes of interest. Transposon is one of the most efficient tools for introducing foreign DNA sequences into host genome. DNA transposon, such as piggyBac, Hermes, Minos, hobo, and mariner, have been identified in insects and have been used successfully as vectors for germline tarsnformation in various insect species. piggyBac-based transformation vectors have been broadly used in generating transgenic silkworm. However, there are few studies reporting vectors for transformation of cultured B. mori cells. In this study, we constructed new piggyBac-based vectors pPigGate, which have visible and drug selectable marker, PuroDsRed or GFPZeo in cultured cells. In order to access the utility of these vectors, we introduced BmHop2 and BmMnd1, which are meiosis specific recombination proteins, into cultured B. mori cells using the pPigGate.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume54
Issue number2
Publication statusPublished - Oct 1 2009

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Morus
Bombyx
drug resistance
silkworms
Drug Resistance
transposons
cultured cells
Insects
Cultured Cells
genetically modified organisms
Genetically Modified Organisms
insects
DNA Transposable Elements
Meiosis
meiosis
Genetic Recombination
Genes
genetic techniques and protocols
germ cells
genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Construction of piggyBac-based vectors using visible and drug-resistance marker for introducing foreign genes into silkworm cultured cells. / Tatsuke, Tsuneyuki; Hong, Sun Mee; Tobata, Hiromi; Mon, Hiroaki; Lee, Jae Man; Kawaguchi, Yutaka; Kusakabe, Takahiro.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 54, No. 2, 01.10.2009, p. 397-400.

Research output: Contribution to journalArticle

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