Construction of rice chromosome specific DNA libraries using chromosome microdissection technique and its application for mapping

K. Ikeda, S. Naka, M. Egashira, Matsumoto Masaru, N. Niikawa, N. Iwata, Atsushi Yoshimura

Research output: Contribution to journalArticle

Abstract

The DNA libraries derived from the entire chromosomes 1, 4, 7 and 9, and the extra chromosomes of acrotrisomics (Acro5a) were constructed using the chromosome microdissection technique. Fluorescence in situ hybridization (FISH) was used to identify the origins of the constructed DNA libraries. DNA libraries from the entire chromosomes 1, 4 and 9 would be available for the identification of chromosome on FISH. In addition, the origin of DNA libraries from the entire chromosome 7 and the extra chromosomes of Acro5a were identified by dot blot hybridization. One hundred sixty and 587 clones were obtained from DNA libraries of the entire chromosomes 1 and 4, respectively. Sixty-three of these clones were surveyed for polymorphism among six varieties. Forty-three unique clones, 35 clones for polymorphism and 20 multicopy clones were obtained. Two of 160 clones and three of 587 clones were mapped on RFLP linkage map using recombinant inbred lines.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number1
Publication statusPublished - Nov 1 2000

Fingerprint

Microdissection
DNA libraries
Gene Library
Clone Cells
Chromosomes
chromosomes
rice
clones
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 9
Fluorescence In Situ Hybridization
fluorescence in situ hybridization
methodology
genetic polymorphism
Chromosomes, Human, Pair 7
Oryza
Restriction Fragment Length Polymorphisms
nucleic acid hybridization
inbred lines

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Construction of rice chromosome specific DNA libraries using chromosome microdissection technique and its application for mapping. / Ikeda, K.; Naka, S.; Egashira, M.; Masaru, Matsumoto; Niikawa, N.; Iwata, N.; Yoshimura, Atsushi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 45, No. 1, 01.11.2000, p. 73-82.

Research output: Contribution to journalArticle

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