Construction of serralysin-like metalloprotease-defcient mutants of Serratia liquefaciens and their virulence in the silkworm, Bombyx mori

Fusako Kaibara, Kazuhiro Iiyama, Yuuka Chieda, Chisa Yasunaga-Aoki, Susumu Shimizu, Jae Man Lee, Takahiro Kusakabe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Serratia liquefaciens FK01 produces two serralysin-like metalloproteases. One of the genes, ser1, has been cloned before. Another gene, designated as ser2, was cloned in this study. The protein encoded by ser2 was a typical serralysin-like metalloprotease, since a zinc binding motif (HEXXHXUGUXH), glycine-rich repeats (GGXGXD), a Met-turn (SXMXY) and an ABC exporter motif (DXXX) were found in the deduced amino acid sequence. In order to investigate the contribution of these proteases to the virulence in an insect, protease- deficient mutants were created. The mutant strains were inoculated to the silkworm, Bombyx mori. The result of the mortality test suggested that we could not show that deficiency in ser1 and ser2 caused a virulence attenuation in the silkworm under our experimental conditions used in this study. It also suggested that other virulence factors existed in the infection in the silkworm.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalJournal of Biotechnology and Sericology
Volume81
Issue number1
DOIs
Publication statusPublished - Jan 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

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