Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium

Ken-ichi Honjoh, Yoshikazu Hashimoto, Satoshi Shimotsu, Hsu Ming Wen, Madoka Kiriki, Kimitaka Naito, Mio Tokugawa, Emiko Satake, Hiroshi Kobayashi, Takahisa Miyamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers.. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and ΔagfA and ΔbcsA mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (ΔahpC, ΔahpF, and ΔkatG mutants of Salmonella Typhimurium, and of ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium) were constructed.

Original languageEnglish
Pages (from-to)421-431
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume54
Issue number2
Publication statusPublished - Oct 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Fingerprint Dive into the research topics of 'Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured <i>Salmonella: ΔagfA</i> and <i>ΔbcsA</i> mutants of <i>Salmonella</i> enteritidis; <i>ΔahpC</i>, <i>ΔahpF</i>, and <i>ΔkatG</i> mutants of <i>S.</i> Typhimurium; and <i>ΔrpoE</i>, <i>ΔrpoH</i>, and <i>ΔrpoS</i> mutants of <i>S.</i> Enteritidis and <i>S.</i> Typhimurium'. Together they form a unique fingerprint.

  • Cite this