Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium

Ken-ichi Honjoh, Yoshikazu Hashimoto, Satoshi Shimotsu, Hsu Ming Wen, Madoka Kiriki, Kimitaka Naito, Mio Tokugawa, Emiko Satake, Hiroshi Kobayashi, Takahisa Miyamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers.. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and ΔagfA and ΔbcsA mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (ΔahpC, ΔahpF, and ΔkatG mutants of Salmonella Typhimurium, and of ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium) were constructed.

Original languageEnglish
Pages (from-to)421-431
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume54
Issue number2
Publication statusPublished - Oct 1 2009

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Salmonella enteritidis
Gene Deletion
Salmonella Enteritidis
Biofilms
Salmonella Typhimurium
Salmonella
biofilm
Hot Temperature
Bacteria
heat
mutants
Genes
Food
bacteria
Foodborne Diseases
genes
Salmonella typhimurium
Plasmids
foodborne illness
microbial growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium",
abstract = "Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers.. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and ΔagfA and ΔbcsA mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (ΔahpC, ΔahpF, and ΔkatG mutants of Salmonella Typhimurium, and of ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium) were constructed.",
author = "Ken-ichi Honjoh and Yoshikazu Hashimoto and Satoshi Shimotsu and Wen, {Hsu Ming} and Madoka Kiriki and Kimitaka Naito and Mio Tokugawa and Emiko Satake and Hiroshi Kobayashi and Takahisa Miyamoto",
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T1 - Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium

AU - Honjoh, Ken-ichi

AU - Hashimoto, Yoshikazu

AU - Shimotsu, Satoshi

AU - Wen, Hsu Ming

AU - Kiriki, Madoka

AU - Naito, Kimitaka

AU - Tokugawa, Mio

AU - Satake, Emiko

AU - Kobayashi, Hiroshi

AU - Miyamoto, Takahisa

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers.. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and ΔagfA and ΔbcsA mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (ΔahpC, ΔahpF, and ΔkatG mutants of Salmonella Typhimurium, and of ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium) were constructed.

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