Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125

Yoshio Hashimoto, Kiyoshi Tanigawa, Manabu Nakashima, Kenzo Sonoda, Tadashi Ueda, Takeshi Watanabe, Taiji Imoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The variable regions of heavy- and light-chains of mouse monoclonal antibody 196-14 toward ovarian cancer-associated antigen CA125 were linked with a peptide linker (GSTSGSGKSSEGKG) and a histidine tag was attached at the carboxyl terminal. This single-chain Fv (scFv) with a histidine tag was expressed in Escherichia coli as an inclusion body. The inclusion body was solubilized with guanidium chloride, followed by purification on nickel nitrilotriacetic acid agarose column and refolding into the active form. The scFv thus obtained bound to the Siso cells, which express CA125, and may recognize the same epitope as the parental 196-14 antibody IgG does.

Original languageEnglish
Pages (from-to)1068-1072
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 1999

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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