Continuous butanol fermentation from xylose with high cell density by cell recycling system

Jin Zheng; Yukihiro Tashiro; Tsuyoshi Yoshida; Ming Gao; Qunhui Wang; Kenji Sonomoto

doi:10.1016/j.biortech.2012.11.066

Continuous butanol fermentation from xylose with high cell density by cell recycling system

Jin Zheng, Yukihiro Tashiro, Tsuyoshi Yoshida, Ming Gao, Qunhui Wang, Kenji Sonomoto

Systems Biology

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26h^-1, indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4gL^-1, which increased butanol productivity to 1.20gL^-1h^-1 at a dilution rate of 0.26h^-1 from 0.529gL^-1h^-1 without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32gL^-1h^-1) was observed at a dilution rate of 0.78h^-1, approximately 6-fold higher than observed in continuous culture without cell recycling (0.529gL^-1h^-1).

Original language	English
Pages (from-to)	360-365
Number of pages	6
Journal	Bioresource Technology
Volume	129
DOIs	https://doi.org/10.1016/j.biortech.2012.11.066
Publication status	Published - Jan 1 2013

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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title = "Continuous butanol fermentation from xylose with high cell density by cell recycling system",

abstract = "A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26h-1, indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4gL-1, which increased butanol productivity to 1.20gL-1h-1 at a dilution rate of 0.26h-1 from 0.529gL-1h-1 without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32gL-1h-1) was observed at a dilution rate of 0.78h-1, approximately 6-fold higher than observed in continuous culture without cell recycling (0.529gL-1h-1).",

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AU - Zheng, Jin

AU - Tashiro, Yukihiro

AU - Yoshida, Tsuyoshi

AU - Gao, Ming

AU - Wang, Qunhui

AU - Sonomoto, Kenji

PY - 2013/1/1

Y1 - 2013/1/1

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AB - A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26h-1, indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4gL-1, which increased butanol productivity to 1.20gL-1h-1 at a dilution rate of 0.26h-1 from 0.529gL-1h-1 without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32gL-1h-1) was observed at a dilution rate of 0.78h-1, approximately 6-fold higher than observed in continuous culture without cell recycling (0.529gL-1h-1).

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