Continuous production of human erythropoietin by immobilized recombinant L-929 cells

Shuji Terashima, Masamichi Kamihira, Tatsuya Ogawa, Masanao Ohno, Shinji Iijima, Takeshi Kobayashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

Original languageEnglish
Pages (from-to)52-56
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume77
Issue number1
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

Erythropoietin
Bioreactors
Productivity
Immobilized Cells
Serum-Free Culture Media
Air
Fluidized beds
Genes
Cells
Derivatives
Polyethyleneimine
Immobilization
Cell Count
Serum

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Continuous production of human erythropoietin by immobilized recombinant L-929 cells. / Terashima, Shuji; Kamihira, Masamichi; Ogawa, Tatsuya; Ohno, Masanao; Iijima, Shinji; Kobayashi, Takeshi.

In: Journal of Fermentation and Bioengineering, Vol. 77, No. 1, 01.01.1994, p. 52-56.

Research output: Contribution to journalArticle

Terashima, Shuji ; Kamihira, Masamichi ; Ogawa, Tatsuya ; Ohno, Masanao ; Iijima, Shinji ; Kobayashi, Takeshi. / Continuous production of human erythropoietin by immobilized recombinant L-929 cells. In: Journal of Fermentation and Bioengineering. 1994 ; Vol. 77, No. 1. pp. 52-56.
@article{5d8fbde8377645f3ada583b122d0d7eb,
title = "Continuous production of human erythropoietin by immobilized recombinant L-929 cells",
abstract = "Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.",
author = "Shuji Terashima and Masamichi Kamihira and Tatsuya Ogawa and Masanao Ohno and Shinji Iijima and Takeshi Kobayashi",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0922-338X(94)90208-9",
language = "English",
volume = "77",
pages = "52--56",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "1",

}

TY - JOUR

T1 - Continuous production of human erythropoietin by immobilized recombinant L-929 cells

AU - Terashima, Shuji

AU - Kamihira, Masamichi

AU - Ogawa, Tatsuya

AU - Ohno, Masanao

AU - Iijima, Shinji

AU - Kobayashi, Takeshi

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

AB - Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

UR - http://www.scopus.com/inward/record.url?scp=0028355068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028355068&partnerID=8YFLogxK

U2 - 10.1016/0922-338X(94)90208-9

DO - 10.1016/0922-338X(94)90208-9

M3 - Article

AN - SCOPUS:0028355068

VL - 77

SP - 52

EP - 56

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 1

ER -