Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells

Takaaki Tanaka, Keiichi Yamamoto, Sirintornthep Towprayoon, Hiroki Nakajima, Kenji Sonomoto, Kenzo Yokozeki, Koji Kubota, Atsuo Tanaka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days.

Original languageEnglish
Pages (from-to)564-568
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 1 1989

Fingerprint

Corynebacterium
Serine
Gels
Glycine
Fermentation
Polysaccharides
Air
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Tanaka, T., Yamamoto, K., Towprayoon, S., Nakajima, H., Sonomoto, K., Yokozeki, K., ... Tanaka, A. (1989). Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells. Applied Microbiology and Biotechnology, 30(6), 564-568. https://doi.org/10.1007/BF00255360

Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells. / Tanaka, Takaaki; Yamamoto, Keiichi; Towprayoon, Sirintornthep; Nakajima, Hiroki; Sonomoto, Kenji; Yokozeki, Kenzo; Kubota, Koji; Tanaka, Atsuo.

In: Applied Microbiology and Biotechnology, Vol. 30, No. 6, 01.06.1989, p. 564-568.

Research output: Contribution to journalArticle

Tanaka, T, Yamamoto, K, Towprayoon, S, Nakajima, H, Sonomoto, K, Yokozeki, K, Kubota, K & Tanaka, A 1989, 'Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells', Applied Microbiology and Biotechnology, vol. 30, no. 6, pp. 564-568. https://doi.org/10.1007/BF00255360
Tanaka, Takaaki ; Yamamoto, Keiichi ; Towprayoon, Sirintornthep ; Nakajima, Hiroki ; Sonomoto, Kenji ; Yokozeki, Kenzo ; Kubota, Koji ; Tanaka, Atsuo. / Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells. In: Applied Microbiology and Biotechnology. 1989 ; Vol. 30, No. 6. pp. 564-568.
@article{0c2aa9ce09fc402497e7653d6657940a,
title = "Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells",
abstract = "Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days.",
author = "Takaaki Tanaka and Keiichi Yamamoto and Sirintornthep Towprayoon and Hiroki Nakajima and Kenji Sonomoto and Kenzo Yokozeki and Koji Kubota and Atsuo Tanaka",
year = "1989",
month = "6",
day = "1",
doi = "10.1007/BF00255360",
language = "English",
volume = "30",
pages = "564--568",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells

AU - Tanaka, Takaaki

AU - Yamamoto, Keiichi

AU - Towprayoon, Sirintornthep

AU - Nakajima, Hiroki

AU - Sonomoto, Kenji

AU - Yokozeki, Kenzo

AU - Kubota, Koji

AU - Tanaka, Atsuo

PY - 1989/6/1

Y1 - 1989/6/1

N2 - Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days.

AB - Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days.

UR - http://www.scopus.com/inward/record.url?scp=0024524609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024524609&partnerID=8YFLogxK

U2 - 10.1007/BF00255360

DO - 10.1007/BF00255360

M3 - Article

VL - 30

SP - 564

EP - 568

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 6

ER -